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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Updated: Sep 1, 2025

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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A CRISPR upgrade unlocks Toxoplasma gene function.

Aurelie Tsee Dawson1, Christopher J Tonkin1

  • 1The Division of Infectious Diseases and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, Victoria, Australia; Department of Medical Biology, The University of Melbourne, Parkville, Melbourne, Victoria, Australia.

Trends in Parasitology
|August 16, 2022
PubMed
Summary
This summary is machine-generated.

Forward genetic screens in Toxoplasma are enhanced by combining CRISPR technology with conditional gene regulation. This breakthrough enables deeper understanding of gene function in this important model parasite.

Keywords:
ApicomplexaCRISPRToxoplasmaauxin-inducible degronphenotypic screeningsplit Cas9

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Area of Science:

  • Genetics
  • Molecular Biology
  • Parasitology

Background:

  • Forward genetic screens are essential for elucidating gene function.
  • CRISPR technology has significantly advanced phenotypic screening capabilities.
  • Toxoplasma gondii is a widely studied model organism in the Apicomplexa phylum.

Purpose of the Study:

  • To introduce and validate a novel approach for forward genetic screening in Toxoplasma.
  • To combine conditional gene regulation with CRISPR for enhanced genetic analysis.
  • To expand the toolkit for functional genomics in apicomplexan parasites.

Main Methods:

  • Utilized CRISPR-based screening methodologies.
  • Implemented conditional gene regulation systems.
  • Performed phenotypic analysis in Toxoplasma gondii.

Main Results:

  • Successfully combined conditional gene regulation with CRISPR for forward genetic screens.
  • Demonstrated the feasibility of this integrated approach in Toxoplasma.
  • Opened new avenues for dissecting gene function in the parasite.

Conclusions:

  • The integration of conditional gene regulation and CRISPR represents a significant advancement in Toxoplasma genetics.
  • This powerful combination facilitates high-throughput functional genomics studies.
  • Future research can leverage this method to uncover novel gene functions and biological pathways in apicomplexans.