Related Experiment Video
Updated: Sep 1, 2025
08:58
Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
12.4K
Mannose ameliorates experimental colitis by protecting intestinal barrier integrity.
Lijun Dong1,2,3, Jingwen Xie2,3, Youyi Wang2,3
1The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, 510900, China.
Nature Communications
|August 16, 2022
Summary
Mannose supplementation shows promise for treating inflammatory bowel disease (IBD). This study found elevated mannose levels in IBD patients and demonstrated mannose
Area of Science:
- Gastroenterology and Immunology
- Metabolomics
- Molecular Biology
Background:
- Metabolite alterations are implicated in inflammatory bowel disease (IBD) pathogenesis.
- Mannose, a monosaccharide, possesses anti-inflammatory and anti-oxidative properties.
- Elevated circulating mannose levels are observed in IBD patients and experimental colitis models.
Purpose of the Study:
- To investigate the therapeutic potential of mannose in colitis models.
- To elucidate the mechanisms underlying mannose's protective effects on the intestinal barrier.
Main Methods:
- Assessment of circulating mannose levels in IBD patients and colitis mouse models.
- Administration of mannose in dextran sodium sulfate (DSS)-induced and IL-10-deficient spontaneous colitis models.
- Analysis of lysosomal integrity, cathepsin B release, mitochondrial function, and tight junction integrity.
- Evaluation of synergistic effects with mesalamine.
Main Results:
- Mannose treatment attenuated intestinal barrier damage in both colitis models.
- Mannose enhanced lysosomal integrity, limited cathepsin B release, and prevented mitochondrial dysfunction.
- Mannose inhibited myosin light chain kinase (MLCK)-induced tight junction disruption.
- Mannose demonstrated a synergistic therapeutic effect with mesalamine.
Conclusions:
- Mannose supplementation may offer a therapeutic strategy for colitis and related conditions.
- Mannose protects the intestinal barrier by preserving lysosomal and mitochondrial integrity.
- Mannose's mechanisms involve mitigating MLCK-induced tight junction disruption.
