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Related Experiment Video

Updated: Sep 1, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Improved ClickTags enable live-cell barcoding for highly multiplexed single cell sequencing.

Xinlu Zhao¹, Shiming Sun¹, Wenhao Yu¹

¹State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University Nanjing Jiangsu China jieli@nju.edu.cn.

RSC Chemical Biology

|August 17, 2022

Summary

This summary is machine-generated.

New ClickTags enable live cell barcoding for single-cell RNA sequencing (scRNA-seq) multiplexing. This method efficiently labels live cells, reducing costs and batch effects in diverse sample types.

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Last Updated: Sep 1, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Published on: April 14, 2015

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Click chemistry DNA barcoding is vital for multiplexing in single-cell RNA sequencing (scRNA-seq).
Existing ClickTags are restricted to fixed samples, limiting their application to live cells.
There is a need for methods that can barcode live cells without altering their physiological state.

Purpose of the Study:

To develop an improved ClickTag protocol for efficient barcoding of live cells.
To enable sample multiplexing of live cells for scRNA-seq.
To demonstrate the broad applicability of the new protocol across different sample types and species.

Main Methods:

Development of optimized ClickTag chemistry for live cell labeling.
Application of the protocol to murine and human primary cells.
Single-cell RNA sequencing (scRNA-seq) for multiplexed sample analysis.

Main Results:

The optimized protocol successfully barcoded live cells without physiological perturbation.
High consistency was achieved in multiplexing and demultiplexing up to 16 samples from murine and human sources.
The method demonstrated general applicability across diverse primary sample types.

Conclusions:

The new ClickTag protocol enables efficient live cell barcoding for scRNA-seq.
This advancement facilitates sample multiplexing, increasing throughput and reducing costs.
The method is valuable for large-scale studies, particularly with clinical samples, by mitigating batch effects.