Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.5K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.5K
Comparing Intermolecular Forces: Melting Point, Boiling Point, and Miscibility02:34

Comparing Intermolecular Forces: Melting Point, Boiling Point, and Miscibility

45.2K
Intermolecular forces are attractive forces that exist between molecules. They dictate several bulk properties, such as melting points, boiling points, and solubilities (miscibilities) of substances. Molar mass, molecular shape, and polarity affect the strength of different intermolecular forces, which influence the magnitude of physical properties across a family of molecules.
Temporary attractive forces like dispersion are present in all molecules, whether they are polar or nonpolar. They...
45.2K
Fluid Mosaic Model01:19

Fluid Mosaic Model

12.4K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
12.4K
The Fluid Mosaic Model01:34

The Fluid Mosaic Model

150.3K
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.
150.3K
Membrane Fluidity01:23

Membrane Fluidity

155.0K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
155.0K
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

80
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
80

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of pH and Protein Flexibility on the Structure of Protein Foams: A Multi-Scale Approach.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Lipids and lipid nanoparticles functionalized with randomized poly(ethylene glycol) (rPEG) for mRNA delivery.

Chemical science·2026
Same author

Quantifying Surfactant Adsorption at Fluid Interfaces by Combining X-ray Reflectivity and Simulations.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Structural characterization of phosphatidylcholine lipid monolayers across the liquid-expanded/liquid-condensed phase transition.

Soft matter·2026
Same author

Temperature- and Pressure-Induced Ligand Anisotropy Drives Structural Reorganization of Dendronized Gold Nanoparticle Monolayers.

Journal of the American Chemical Society·2026
Same author

RNA at Lipid/Water Interfaces: Molecular Insights from Coarse-Grained Simulations and Reflectivity Data Predictions.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Sep 1, 2025

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.3K

Phase Behavior and Miscibility in Two-Component Glycolipid Monolayers.

Tetiana Mukhina1, Gerald Brezesinski1, Emanuel Schneck1

  • 1Institute for Condensed Matter Physics, TU Darmstadt, Hochschulstraße 8, 64289 Darmstadt, Germany.

The Journal of Physical Chemistry. B
|August 17, 2022
PubMed
Summary

Binary glycolipid mixtures form complex membrane domains. Headgroup chemistry dictates whether ordered structures are conserved or lost, impacting biological membrane behavior.

More Related Videos

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.6K
Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
10:11

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

3.8K

Related Experiment Videos

Last Updated: Sep 1, 2025

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.3K
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.6K
Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
10:11

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

3.8K

Area of Science:

  • Biochemistry
  • Materials Science
  • Membrane Biophysics

Background:

  • Glycolipids are crucial for ordered functional domains in biological membranes.
  • Characterizing these membrane domains is challenging, often limiting studies to single glycolipid components.
  • Biological membranes typically contain multiple glycolipid species, leading to complex structures and phase behavior.

Purpose of the Study:

  • Investigate the phase behavior and miscibility of binary glycolipid mixtures.
  • Understand how saccharide headgroup chemistry influences the formation and stability of membrane domains.
  • Explore the potential for molecular superlattices to host foreign glycolipids.

Main Methods:

  • Langmuir monolayer formation.
  • Classical isotherm measurements.
  • Surface-sensitive grazing-incidence X-ray diffraction (GIXD).

Main Results:

  • Phase behavior is subtly dependent on saccharide headgroup chemistry.
  • Compatible glycolipid chemistries conserve molecular superlattice structures.
  • Sterically incompatible chemistries disrupt superlattices, even if components form them individually.
  • Superlattices can host foreign glycolipids up to a specific stoichiometry.

Conclusions:

  • Saccharide headgroup chemistry is a key determinant of glycolipid mixture phase behavior.
  • Molecular superlattices in glycolipid mixtures exhibit selective hosting capabilities.
  • These findings have implications for understanding complex lipid organization in biological membranes.