Related Experiment Video
Updated: Sep 1, 2025

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
2-Oxo-imidazole dipeptides in meat.
Somei Komae1, Shingo Kasamatsu1,2, Hideshi Ihara1,2
1Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka, Japan.
This study found that 2-oxo-imidazole dipeptides (2-oxo-IDPs) are present in meat and their levels vary by animal and body part. Aeration can increase 2-oxo-IDPs in chicken, suggesting potential meat applications.
Area of Science:
- Food Chemistry
- Biochemistry
Background:
- 2-Oxo-imidazole dipeptides (2-oxo-IDPs) are known for their functionality.
- Their presence and levels in meat products remain largely uncharacterized.
Purpose of the Study:
- To determine the existence and levels of 2-oxo-IDPs in various meat types.
- To investigate factors influencing 2-oxo-IDP concentrations in meat.
Main Methods:
- Quantification of 2-oxo-IDPs in different animal species and body parts.
- Analysis of 2-oxo-IDP changes in chicken breast extract under specific conditions (aeration, CuSO4/ascorbate).
Main Results:
- 2-Oxo-IDPs were detected in meat, with levels varying significantly based on animal species and anatomical location.
- Aeration in the presence of CuSO4/ascorbate led to an increase in 2-oxo-IDPs in chicken breast extract.
Conclusions:
- Meat contains 2-oxo-IDPs, and their distribution is not uniform across species or tissues.
- The findings suggest that meat processing conditions can modulate 2-oxo-IDP levels, opening possibilities for enhanced meat utilization.
More Related Videos
Related Concept Videos
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Dipeptidyl Peptidase 4 Inhibitors
Protein Digestion

