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Updated: Sep 1, 2025

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
Published on: January 22, 2013
Papillary renal neoplasm with reverse polarity-a comparative study with CCPRCC, OPRCC, and PRCC1
Tao Wang1, Xiaohui Ding2, Xing Huang1
1Department of Urology, The Third Medical Centre, Chinese People's Liberation Army (PLA) General Hospital, Beijing, 100039, China; Medical School of Chinese People's Liberation Army (PLA), Beijing, 100039, China.
Papillary renal neoplasm with reverse polarity (PRNRP) is a distinct kidney tumor. This study characterized PRNRP, differentiating it from similar kidney cancers using histopathology, IHC, and molecular analysis.
Area of Science:
- Uropathology
- Renal Neoplasms
- Molecular Pathology
Background:
- Papillary renal neoplasm with reverse polarity (PRNRP) is a recently identified renal tumor.
- Distinct histomorphology and KRAS mutations characterize PRNRP.
Purpose of the Study:
- To analyze clinicopathological, immunohistochemical (IHC), and molecular features of PRNRP.
- To differentiate PRNRP from clear cell papillary renal cell carcinoma (CCPRCC), oncocytic papillary renal cell carcinoma (OPRCC), and papillary renal cell carcinoma type 1 (PRCC1).
Main Methods:
- Retrospective analysis of nephrectomy specimens (PRNRP, CCPRCC, OPRCC, PRCC1).
- Immunohistochemical (IHC) staining for specific markers.
- KRAS mutation analysis and broader gene mutation profiling.
Main Results:
- PRNRP constituted 3.05% of papillary renal cell carcinoma cases.
- PRNRP exhibited distinct IHC features: diffuse CK7, EMA, GATA3; weak/negative CD10, CD117, p504s, vimentin.
- KRAS mutations were found in 40% of PRNRP cases; 5 actionable alterations identified.
Conclusions:
- PRNRP is a distinct entity separate from CCPRCC, OPRCC, and PRCC1.
- Clinicopathological, IHC, and molecular findings support PRNRP's unique classification.
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