Fluorescence Imaging of 3D Cell Models with Subcellular Resolution.

Area of Science:

• Biotechnology
• Cell Biology
• Microscopy

Background:

• 3D cell models offer superior *in vivo* physiological representation compared to 2D cultures.
• Applications include drug development, cancer biology, and personalized medicine.
• Advanced imaging techniques are crucial for extracting insights from 3D models.

Purpose of the Study:

• To provide standardized protocols for sample preparation and fluorescence imaging of 3D cell models.
• To detail guidelines for confocal multi-photon fluorescence microscopy of these complex structures.

Main Methods:

• Development of sample preparation protocols for both matrix-embedded and matrix-free 3D cell cultures (organoids, spheroids).
• Establishment of imaging guidelines for confocal multi-photon fluorescence microscopy.

Main Results:

• Successful acquisition of high-resolution fluorescence images from diverse 3D cell models.
• Demonstration of sub-cellular resolution imaging capabilities using the presented protocols.

Conclusions:

• The provided protocols facilitate robust fluorescence imaging of 3D cell models.
• These methods enhance the utility of 3D cell cultures for biological research and drug discovery.