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Published on: January 7, 2019
Preparation of Drosophila Tissues and Organs for Transmission Electron Microscopy
Olympia-Ekaterini Psathaki1,2, Achim Paululat3
1University of Osnabrück, Department of Zoology and Developmental Biology, Osnabrück, Germany.
Abstract:
Transmission electron microscopy (TEM) is the method of choice to image the ultrastructure of cells or tissues. TEM allows the visualization of molecular complexes up to an atomic resolution. Thus, TEM data have led to important conclusions about cellular processes and supported findings obtained by functional analyses. In this chapter, we describe the preparation of Drosophila tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing-freeze substitution (HPF-FS) to preserve cellular structures.

