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[Melatonin Induced Apoptosis of RPMI 8226 Cells through Endoplasmic Reticulum Stress].

Ting Chen1, Li-Cheng Li1, Yan Zhang1

  • 1Department of Hematology, Guizhou Medical University Affiliated Hospital, Guiyang 550004, Guizhou Province, China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi
|August 18, 2022
PubMed
Summary
This summary is machine-generated.

Melatonin (MLT) significantly inhibits multiple myeloma cell proliferation and promotes apoptosis. This research suggests MLT enhances bortezomib

Keywords:
apoptosisendoplasmic reticulum stressmelatoninmyeloma cells

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Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Multiple myeloma is a hematological malignancy characterized by uncontrolled plasma cell proliferation.
  • Melatonin is a hormone with potential anti-cancer properties.
  • Understanding novel therapeutic strategies for multiple myeloma is crucial.

Purpose of the Study:

  • To investigate the effects of melatonin (MLT) on the proliferation and apoptosis of the human multiple myeloma cell line RPMI 8226.
  • To elucidate the underlying mechanisms, including endoplasmic reticulum stress.
  • To evaluate the combined effect of MLT and bortezomib (BTZ).

Main Methods:

  • In vitro culture of RPMI 8226 cells with varying MLT concentrations.
  • Cell proliferation assessed by CCK-8 and methylcellulose cloning assays.
  • Apoptosis evaluated using AnnexinV-FITC/PI and flow cytometry.
  • Western blot analysis for apoptosis and endoplasmic reticulum stress markers.
  • Combined MLT and bortezomib treatment viability assay.

Main Results:

  • MLT demonstrated dose- and time-dependent inhibition of RPMI 8226 cell proliferation.
  • Increased MLT concentrations led to reduced clone formation and enhanced apoptosis (P<0.05).
  • MLT modulated apoptosis-related proteins (XIAP, Bax, Caspase3) and increased endoplasmic reticulum stress markers.
  • Combined MLT and bortezomib significantly reduced cell survival (P<0.01).

Conclusions:

  • Melatonin effectively inhibits multiple myeloma cell proliferation and induces apoptosis.
  • MLT enhances the anti-tumor efficacy of bortezomib against RPMI 8226 cells.
  • The mechanism of action may involve the induction of endoplasmic reticulum stress.