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Updated: Aug 31, 2025

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
[Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples]
Hui Ni Huang1, Zhu Ning Mo1, Xiang Cheng Liao1
1Department of Blood Transfusion, Guangxi Zhuang Autonomous Region People's Hospital, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
Objective:
Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.
Methods:
A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.
Results:
The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.
Conclusion:
ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.
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