Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Analytical Methods01:21

Development of Analytical Methods

674
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
674
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation02:17

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation

3.4K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.4K
ATP and Macromolecule Synthesis01:28

ATP and Macromolecule Synthesis

6.0K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
6.0K
Qualitative Analysis01:10

Qualitative Analysis

636
Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...
636
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

435
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
435

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<sup>13</sup>C Metabolic Flux Analysis and Gene Expression Analysis of Perfusion Culture Reveal Metabolic Bottlenecks for Stable Glycosylation Profiles of Biologics.

Metabolic engineering·2026
Same author

In Situ Microscopy for Real-Time Visualization of Microcarrier Cell Cultures for Live Virus Vaccine Process Development.

Biotechnology and bioengineering·2026
Same author

Elucidating the Critical Attributes of Sodium Triacetoxyborohydride to Tune Glycoconjugation via Reductive Amination.

Bioconjugate chemistry·2025
Same author

Investigating Interfacial Interactions between the Adjuvant and Antigens in Pneumococcal Conjugate Vaccine In Situ at the Molecular Level.

Analytical chemistry·2025
Same author

Real-time monitoring of attenuated cytomegalovirus using Raman spectroscopy allows non-destructive characterization during flow.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same author

Dermatological Emergencies in Critical Care.

Annals of the American Thoracic Society·2025

Related Experiment Video

Updated: Aug 31, 2025

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

9.8K

Process analytical technology and its recent applications for asymmetric synthesis.

Nicole M Ralbovsky1, Joseph P Smith1

  • 1Analytical Research & Development, MRL, Merck & Co., Inc., West Point, PA 19486, USA.

Talanta
|August 18, 2022
PubMed
Summary

Process analytical technology (PAT) enhances the development of chiral active pharmaceutical ingredients (APIs) by enabling real-time monitoring and control of asymmetric syntheses, crucial for pharmaceutical manufacturing.

Keywords:
Active pharmaceutical ingredientAsymmetric synthesisChiral moleculesPharmaceuticalsProcess analytical technologyReal time monitoring

More Related Videos

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

961
Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
08:24

Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling

Published on: November 11, 2008

16.5K

Related Experiment Videos

Last Updated: Aug 31, 2025

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

9.8K
Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

961
Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
08:24

Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling

Published on: November 11, 2008

16.5K

Area of Science:

  • Pharmaceutical Science
  • Organic Chemistry
  • Chemical Engineering

Background:

  • Active pharmaceutical ingredient (API) development is critical in the pharmaceutical industry.
  • Chiral compounds are frequently encountered in API synthesis, often requiring asymmetric synthesis routes.
  • Asymmetric syntheses demand meticulous control for efficiency and success.

Purpose of the Study:

  • To review the applications of Process Analytical Technology (PAT) in asymmetric synthesis.
  • To highlight how PAT aids in investigating and optimizing chiral compound generation.
  • To provide a repository of recent advancements in PAT for pharmaceutical chiral synthesis.

Main Methods:

  • Review of literature on PAT applications in asymmetric synthesis.
  • Analysis of PAT tools and techniques for real-time process monitoring.
  • Discussion of case studies demonstrating PAT implementation in chiral synthesis.

Main Results:

  • PAT offers significant advantages for real-time monitoring, control, and assessment of synthetic processes.
  • Implementation of PAT can lead to improved efficiency, yield, and enantioselectivity in asymmetric reactions.
  • PAT facilitates a deeper understanding of reaction mechanisms and critical process parameters.

Conclusions:

  • PAT is a valuable tool for advancing the pharmaceutical industry's capability to produce chiral compounds.
  • The integration of PAT into asymmetric synthesis workflows is essential for robust and scalable API manufacturing.
  • Continued research and application of PAT will drive innovation in chiral drug development.