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Updated: Aug 31, 2025
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
Published on: January 22, 2015
The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles
Chaojie Xu1, Ronge Xing2, Song Liu2
1CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao 266237, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
In this study, nanoparticles were prepared by using positively charged quaternized chitosan and negatively charged mucopolysaccharide such as chondroitin sulfate, heparin and hyaluronic acid. The nanoparticles have a stable nanostructure with particle size in 336.2-424.5 nm, potential in 18.5-31.1 mV and polydispersity index PDI of 0.172-0.335. Moreover, their encapsulation efficiency was 68.77 % and 64.89 %, and they have low endotoxin and good stability. It can significantly promote the expression of IL-6, TNF-α, and IL-1β of DCS cells. Moreover, the in vivo immune activity of heparin-quaternized chitosan-OVA nanoparticles against BALB/C mice was showed that, the nanoparticles could significantly promote the secretion of immunoglobulins in mice including IgG1 and IgG2. And nanoparticle also can promote the production of immune factors. Meanwhile, the expression of immune factor genes was also elevated. Furthermore, the results of tissue section experiments showed that the nanoparticles are safety of the body.
