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Summary

Image-based plate readers, like ViewLux and FLIPR, offer a faster method for NanoBRET assays, enabling high-throughput screening and kinetics studies by capturing entire plates simultaneously.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • NanoBRET assays are crucial for drug discovery, measuring protein-protein interactions and target engagement.
  • Traditional plate readers are time-consuming for high-throughput applications.

Purpose of the Study:

  • To adapt image-based plate readers (ViewLux, FLIPR) for NanoBRET signal detection.
  • To assess the performance of image-based readers compared to traditional methods.

Main Methods:

  • Utilized NanoBRET assays with tool compounds.
  • Employed image-based plate readers with CCD cameras for simultaneous plate imaging.
  • Compared data with traditional well-scanning readers (e.g., EnVision).

Main Results:

  • Image-based plate readers successfully measured NanoBRET signals with high signal-to-background (S/B) and Z´ values.
  • Comparable IC50 values were obtained from image-based and traditional readers.
  • Image-based readers significantly reduced assay read times.

Conclusions:

  • Image-based plate readers are suitable for NanoBRET measurements.
  • This adaptation enhances throughput for drug discovery applications like screening and kinetics.