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Related Experiment Videos

Thermotolerance induced by 2,4-dinitrophenol.

E K Boon-Niermeijer, J E Souren, R Van Wijk

    International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group
    |March 1, 1987
    PubMed
    Summary
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    2,4-dinitrophenol (DNP) can induce short-term thermotolerance in Lymnaea larvae. This effect, optimal at specific conditions, does not involve heat shock protein synthesis.

    Area of Science:

    • * Molecular biology
    • * Environmental toxicology
    • * Invertebrate physiology

    Background:

    • * Organisms face environmental stressors, necessitating adaptive mechanisms like thermotolerance.
    • * Heat shock proteins (HSPs) are commonly associated with cellular stress responses and thermotolerance.
    • * Investigating alternative pathways for thermotolerance induction is crucial for understanding cellular defense.

    Purpose of the Study:

    • * To investigate the capacity of 2,4-dinitrophenol (DNP) to induce thermotolerance in Lymnaea larvae.
    • * To determine the optimal conditions for DNP-induced thermotolerance.
    • * To elucidate the relationship between DNP treatment, protein synthesis, and heat shock protein production.

    Main Methods:

    • * Exposure of 3-day-old Lymnaea larvae to varying concentrations and durations of 2,4-dinitrophenol (DNP) at different pH levels.

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  • * Measurement of thermotolerance through survival percentages.
  • * Assessment of protein synthesis inhibition and heat shock protein (HSP) synthesis following DNP treatment.

    • Main Results:

    • * DNP effectively induces thermotolerance in Lymnaea larvae, with optimal induction at 10 min, 2.5 mM DNP, and pH 4.7.
    • * Thermotolerance peaked at 15 minutes post-treatment and subsequently declined.
    • * DNP treatment suppressed protein synthesis and did not trigger the production of typical heat shock proteins (HSPs).

    Conclusions:

    • * 2,4-dinitrophenol (DNP) confers a transient state of thermotolerance in Lymnaea larvae.
    • * The DNP-induced thermotolerance mechanism operates independently of heat shock protein (HSP) synthesis.
    • * This study highlights a non-HSP-dependent pathway for acquiring short-term thermal resistance in invertebrates.