Related Experiment Video
Updated: Aug 31, 2025
Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
Published on: February 17, 2023
Using human iPSC-derived kidney organoids to decipher SARS-CoV-2 pathology on single cell level
Katharina C Reimer1, Jitske Jansen2, Gijs J Overheul3
1Institute of Experimental Medicine and Systems Biology, RWTH Aachen University, Aachen, Germany; Institute of Cell and Tumor Biology, RWTH Aachen University, Aachen, Germany; Division of Nephrology and Clinical Immunology, RWTH Aachen University, Aachen, Germany.
Abstract:
We describe a protocol for single-cell RNA sequencing of SARS-CoV-2-infected human induced pluripotent stem cell (iPSC)-derived kidney organoids. After inoculation of kidney organoids with virus, we use mechanical and enzymatic disruption to obtain single cell suspensions. Next, we process the organoid-derived cells into sequencing-ready SARS-CoV-2-targeted libraries. Subsequent sequencing analysis reveals changes in kidney cells after virus infection. The protocol was designed for kidney organoids cultured in a 6-well transwell format but can be adapted to organoids with different organ backgrounds. For complete details on the use and execution of this protocol, please refer to Jansen et al. (2022).
