Two new terpenoid glycosides from Isodon macrocalyx

Area of Science:

• Natural Product Chemistry
• Pharmacology

Background:

• Isodon macrocalyx is a plant source of bioactive compounds.
• Triterpenoids are a class of natural products with diverse biological activities.
• Hepatitis B virus (HBV) infection remains a significant global health concern.

Purpose of the Study:

• To isolate and characterize chemical constituents from Isodon macrocalyx.
• To evaluate the in vitro anti-HBV activity of the isolated compounds.

Main Methods:

• Isolation of compounds using chromatographic techniques.
• Structure elucidation using 1D and 2D Nuclear Magnetic Resonance (NMR) spectroscopy and High-Resolution Electrospray Ionization Mass Spectrometry (HRESIMS).
• In vitro anti-HBV activity assay using HBV transfected Hep G2.2.15 cell line.

Main Results:

• One new tricyclo-diterpenoid saponin (1) and one new dammarane triterpenoid saponin (2) were identified.
• Four known triterpenoids (3-6) were also isolated and characterized.
• Compounds 2 and 3 demonstrated moderate anti-HBV activity.

Conclusions:

• Isodon macrocalyx contains novel and known triterpenoids with potential therapeutic applications.
• Compounds 2 and 3 show promise as starting points for developing new anti-HBV agents.