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Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Induced Pluripotent Stem Cells01:06

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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Embryonic Stem Cells00:57

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Related Experiment Video

Updated: Aug 31, 2025

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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Clinical potential of human amniotic fluid stem cells.

Monique M Martin1, Michael Chan1, Clarel Antoine1

  • 1Department of Obstetrics and Gynecology, New York University Langone Health, New York, NY, USA.

Journal of Perinatal Medicine
|August 19, 2022
PubMed
Summary

Human amniotic fluid stem cells retain their stemness after processing at a cell therapy center and simulated clinical application. These findings support potential therapeutic uses for amniotic fluid stem cells.

Keywords:
amniotic fluidcell therapyclinical usestem cells

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Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
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Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Cell therapy

Background:

  • Amniotic fluid is a source of stem cells with therapeutic potential.
  • Processing and clinical application can affect stem cell characteristics.

Purpose of the Study:

  • To evaluate stem cell properties of amniotic fluid-derived stem cells after processing in a clinical setting.
  • To assess cell viability and characteristics after simulated clinical application.

Main Methods:

  • Amniotic fluid collected during cesarean delivery was processed at a licensed cell therapy center.
  • Cells were thawed, passed through a clinical delivery device, and analyzed for viability, stability, growth, differentiation, and stemness markers.

Main Results:

  • Amniotic fluid stem cells processed clinically maintained viability, stability, growth, differentiation, and stemness markers.
  • Cells remained viable for at least 3 hours at room temperature and after passage through a clinical delivery device.

Conclusions:

  • Human amniotic fluid stem cells processed in a clinical facility maintain their essential characteristics.
  • These cells show promise for therapeutic applications, pending safety validation.