A new chromone functionalized isoqunoline derived chemosensor with fluorogenic switching effect for selective...

In this work, a selective chemosensor, (E)-N'-((4-oxo-4H-chromen-3-yl)methylene)isoquinoline-1-carbohydrazide (ENO), was rationally developed for colorimetric and fluorogenic detection of Zn²⁺ ions. It was readily synthesized from 4-oxo-4H-chromene-3-carbaldehyde and isoquinoline-1-carbohydrazide via one-step Schiff reaction. ENO exhibited excellent fluorescent response performances toward Zn²⁺ over a wide pH range in EtOH/H₂O media, including a distinguished color change from colorless to gold, a low limit of detection (LOD) value (34 nM), strong complexation ability (1.36 × 10⁵ M^-1) and rapid identification (2 min). The sensing mechanism of ENO toward Zn²⁺ was proposed on the basis of the chelation-enhanced fluorescence (CHEF) process, which was further supported by IR studies and the density functional theory (DFT) calculation. Moreover, ENO presented here demonstrated outstanding capability in monitoring trace level of Zn²⁺ ions in real water samples, living cells as well as the on-site assay kit.