Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.5K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.5K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.5K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.5K
Chromosome Structure02:40

Chromosome Structure

23.3K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
23.3K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.6K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.6K
DNA Packaging00:58

DNA Packaging

103.3K
Overview
103.3K
Chromatin Packaging02:21

Chromatin Packaging

15.8K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
15.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

KAS-CUT&Tag for direct mapping of transcription bubbles.

bioRxiv : the preprint server for biology·2026
Same author

Superabundant microRNAs are transcribed from human rDNA spacer promoters insulated by CTCF.

Science advances·2026
Same author

RNA polymerase II: the elephant in the room.

Trends in genetics : TIG·2026
Same author

Cell-cycle-dependent repression of histone gene transcription by histone H4.

Nature structural & molecular biology·2026
Same author

An integrated view of the structure and function of the human 4D nucleome.

Nature·2025
Same author

Nascent CUT&Tag captures transcription factor binding after chromatin duplication.

bioRxiv : the preprint server for biology·2025
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
See all related articles

Related Experiment Video

Updated: Aug 31, 2025

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.3K

Centromeres organize (epi)genome architecture.

Paul Talbert1, Steven Henikoff1

  • 1Howard Hughes Medical Institute, Fred Hutchinson Cancer Center, 1100 Fairview Avenue N, Seattle, WA 98109, USA.

Cell
|August 19, 2022
PubMed
Summary
This summary is machine-generated.

Holocentromeres, where microtubules attach along the entire chromosome, influence more than just cell division. In the beak sedge Rhynchospora, they also shape genome structure, epigenetics, and chromosome evolution.

More Related Videos

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.6K
In-Nucleus Hi-C in Drosophila Cells
11:58

In-Nucleus Hi-C in Drosophila Cells

Published on: September 15, 2021

4.2K

Related Experiment Videos

Last Updated: Aug 31, 2025

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.3K
Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.6K
In-Nucleus Hi-C in Drosophila Cells
11:58

In-Nucleus Hi-C in Drosophila Cells

Published on: September 15, 2021

4.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • Mitosis involves chromosome segregation, typically regulated by a single centromere.
  • Some organisms possess holocentromeres, where microtubules attach along the chromosome's length.
  • The impact of holocentromeres on broader genomic and evolutionary aspects remains less understood.

Purpose of the Study:

  • To investigate the role of holocentromeres in the beak sedge Rhynchospora.
  • To determine how holocentromeres influence genomic architecture and epigenome organization.
  • To explore the implications of holocentromeres for karyotype evolution.

Main Methods:

  • Comparative genomics
  • Epigenomic analysis
  • Karyotype analysis in Rhynchospora

Main Results:

  • Holocentromeres in Rhynchospora significantly impact the overall genomic architecture.
  • Evidence suggests holocentromeres play a role in organizing the epigenome.
  • The study provides insights into how holocentromeres contribute to karyotype evolution.

Conclusions:

  • Holocentromeres are key regulators of genome organization and evolution in holocentric organisms.
  • Rhynchospora serves as a model for understanding holocentromere function beyond chromosome segregation.
  • Further research into holocentric systems can reveal novel mechanisms of genome regulation and evolution.