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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
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An optimized protocol for estimating cellulase activity in biological samples.

Zainab Abbas Al Talebi1, Hawraa Saad Al-Kawaz2, Rasha Kadhim Mahdi3

  • 1Chemistry Dept., College of Science, University of Babylon, Hilla City, Babylon Governorate, p.o. 51002, Iraq.

Analytical Biochemistry
|August 19, 2022
PubMed
Summary

A new picric acid (PCA) method accurately quantifies cellulase activity using fewer chemicals. This fast, inexpensive assay is ideal for diverse samples in food and textile industries.

Keywords:
Box–Behnken designCarboxymethylcelluloseCellulase assayMicroplate protocolPicric acid

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Area of Science:

  • Biochemistry
  • Enzymology
  • Analytical Chemistry

Background:

  • Cellulase is a key enzyme degrading cellulose in food and textiles.
  • Existing cellulase activity assays are often complex, costly, or lack sensitivity.
  • There is a need for a reliable, fast, and inexpensive cellulase quantification method.

Purpose of the Study:

  • To develop and validate a novel, sensitive, and cost-effective method for quantifying cellulase activity.
  • To optimize the assay using statistical design and validate its performance against established methods.

Main Methods:

  • Developed a picric acid (PCA) based assay reacting with glucose produced by cellulase.
  • Utilized sodium hydroxide for alkalinity, enhancing picramic acid stability and assay sensitivity.
  • Optimized the PCA-cellulase method using Box-Behnken design and validated with Bland-Altman analysis.

Main Results:

  • The novel PCA-cellulase method demonstrated high sensitivity and linearity.
  • The assay requires fewer chemicals at lower concentrations compared to traditional methods.
  • Validation showed excellent agreement with existing methods, with a correlation coefficient of 0.9991.

Conclusions:

  • The PCA-cellulase method offers an accurate, reliable, and cost-effective alternative for cellulase activity quantification.
  • This method is suitable for various biological and environmental samples, addressing limitations of previous assays.