Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Etiology and pathogens of fungal endophthalmitis].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2015
Same author

Eucommia ulmoides Oliv. bark aqueous extract inhibits osteoarthritis in a rat model of osteoarthritis.

Journal of ethnopharmacology·2015
Same author

Aucubin prevents interleukin-1 beta induced inflammation and cartilage matrix degradation via inhibition of NF-κB signaling pathway in rat articular chondrocytes.

International immunopharmacology·2015
Same author

Treatment with recombinant lubricin attenuates osteoarthritis by positive feedback loop between articular cartilage and subchondral bone in ovariectomized rats.

Bone·2015
Same author

Tet1-mediated DNA demethylation regulates neuronal cell death induced by oxidative stress.

Scientific reports·2015
Same author

Authors' reply.

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association·2015
Same journal

Disruption of the claustrum-ACC pathway contributes to human mind blanking.

Neurobiology of disease·2026
Same journal

Loss of immunological control at CD28 superfamily checkpoints links brain and peripheral immunity to depressive-like behaviors in animal models.

Neurobiology of disease·2026
Same journal

Investigating tau-related white matter degeneration in Alzheimer's disease using fixel-based analysis.

Neurobiology of disease·2026
Same journal

Evidence for hippocampal globotriaosylceramide (Gb3) accumulation and spatial memory impairment in a mouse model of Fabry disease.

Neurobiology of disease·2026
Same journal

Leveraging the dominant-negative effect of the kuru-protective G127V prion protein variant as a novel therapeutic strategy.

Neurobiology of disease·2026
Same journal

GDAP1 orchestrates redox signaling at membrane contact sites to preserve axonal integrity in Charcot-Marie-Tooth disease.

Neurobiology of disease·2026
See all related articles

Related Experiment Video

Updated: Aug 31, 2025

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

27.5K

Aberrant brain functional and structural developments in MECP2 duplication rats.

Ming Xu1, Shile Qi2, Vince Calhoun3

  • 1Brainnetome Center and National Laboratory of Pattern Recognition, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; School of Artificial Intelligence, University of Chinese Academy of Sciences, Beijing, 100190, China.

Neurobiology of Disease
|August 19, 2022
PubMed
Summary
This summary is machine-generated.

MECP2 duplication syndrome in rats shows abnormal brain network development, impacting behavior. This research offers insights into autism spectrum disorder (ASD) neurobiology and potential biomarkers.

Keywords:
Autistic spectrum disorder (ASD)Brain developmentMECP2 duplicationMagnetic resonance imaging (MRI)Multimodal fusionRat brain

More Related Videos

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

8.7K
Strategies for Assessing Autistic-Like Behaviors in Mice
07:38

Strategies for Assessing Autistic-Like Behaviors in Mice

Published on: September 20, 2024

1.3K

Related Experiment Videos

Last Updated: Aug 31, 2025

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

27.5K
A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

8.7K
Strategies for Assessing Autistic-Like Behaviors in Mice
07:38

Strategies for Assessing Autistic-Like Behaviors in Mice

Published on: September 20, 2024

1.3K

Area of Science:

  • Neuroscience
  • Genetics
  • Developmental Biology

Background:

  • Autism spectrum disorder (ASD) symptoms are linked to neurobiological deficits.
  • MECP2 duplication syndrome, caused by MECP2 gain-of-function mutations, presents core ASD symptoms but its underlying brain development is understudied.
  • Transgenic animal models are crucial for investigating the neurobiology of ASD-like behaviors.

Purpose of the Study:

  • To investigate the abnormal brain development and networks associated with autistic-like behaviors in a novel rat model of MECP2 duplication syndrome.
  • To identify potential neuroimaging biomarkers for MECP2 duplication syndrome and ASD.

Main Methods:

  • Created a human MECP2 duplication (MECP2-DP) rat model using bacterial artificial chromosome technology.
  • Utilized high-field functional and structural magnetic resonance imaging (MRI) at multiple postnatal ages (28, 42, and 56 days).
  • Applied multimodal fusion analyses, incorporating locomotor and social novelty metrics, to link brain network abnormalities with behavioral deficits.

Main Results:

  • Identified aberrant functional development in a core network involving the dorsal medial prefrontal cortex (dmPFC) and retrosplenial cortex (RSP) in MECP2-DP rats, correlating with behavioral phenotypes.
  • Detected altered gray matter volume development in the hippocampus and thalamus of MECP2-DP rats.
  • Established associations between MECP2 gain-of-function mutations, aberrant brain network activity, and volumetric changes with autistic-like behaviors.

Conclusions:

  • Gain-of-function MECP2 mutations induce abnormal functional activity in default-mode-like networks and volumetric brain changes, leading to autistic-like behavioral deficits.
  • The MECP2-DP rat model provides critical insights into the neurobiological underpinnings of MECP2 duplication syndrome and ASD.
  • Findings contribute to understanding biomarkers for MECP2 duplication syndrome and the neurobiology of ASD behavioral deficits.