Related Experiment Video
Updated: Aug 31, 2025

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Whole genome sequence analysis of bacteriophage P1 that infects the Lactobacillus plantarum
She Guo1,2,3, Qiannan Wen1,2,3, Jie Zhao1,2,3
1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
Abstract:
Phage P1 was isolated from the abnormal fermented liquid using Lactobacillus plantarum (L. plantarum) IMAU10120. To date, genetic knowledge regarding L. plantarum phage diversity is still limited, and further in-depth sequencing analysis of isolated L. plantarum phages can fill this gap. Here, we investigated the whole genome sequence of L. plantarum phage P1, sequenced by Illumina HiSeq platform, to decipher its genomic characteristics and putative DNA packaging mechanism. It was revealed that phage P1 was 73,787 bp in length, which was composed of linear double-stranded DNA (dsDNA), and the GC content was 39.17%. Its genome contained 86 coding sequences for various functions, such as adsorption, injection, replication, assembly, and release. Moreover, it was observed that L. plantarum phage P1 utilized the 'cohesive ends' DNA packaging mechanism. This study furthered the genomic knowledge of L. plantarum phages and provided some basis for the control of L. plantarum phages in the dairy fermentation industry.
Related Concept Videos
DNA Bacteriophages
Lytic Cycle of Bacteriophages
Viral Replication: Lytic Cycle
Lysogenic Cycle of Bacteriophages
Viral Replication: Lysogenic Cycle

