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Conventional optimization and characterization of microbial dextran using treated sugarcane molasses.

Sameeha Syed Abdul Rahman1, Saroja Pasupathi1, Sugumaran Karuppiah1

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Summary

This study optimized microbial dextran production using treated sugarcane molasses (SCM). The best SCM treatment method yielded 17.18 g/L dextran, with further analysis of its structure and non-Newtonian properties.

Keywords:
DextranStructural characterizationSugarcane molasses

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Area of Science:

  • Biotechnology
  • Microbial Fermentation
  • Biopolymer Production

Background:

  • Sugarcane molasses (SCM) is a potential low-cost feedstock for microbial dextran production.
  • Optimizing SCM treatment is crucial for efficient dextran fermentation.
  • Limited research exists on comparing different SCM treatment strategies for microbial dextran synthesis.

Purpose of the Study:

  • To compare the efficacy of various SCM treatment methods for microbial dextran production.
  • To optimize fermentation parameters for maximizing dextran yield.
  • To characterize the structure and rheological properties of dextran produced from SCM.

Main Methods:

  • SCM was subjected to different pre-treatment methods.
  • Microbial fermentation was conducted using optimized conditions (sugar concentration, nitrogen source, inoculum size, pH, shaking speed, phosphate source).
  • Dextran yield was quantified, and structural analysis was performed using Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. Rheological behavior was assessed.

Main Results:

  • The optimal SCM treatment method was identified based on dextran yield.
  • Conventional optimization yielded a maximum dextran concentration of 17.18 ± 0.08 g/L.
  • Structural analysis confirmed dextran production, and rheological studies indicated a non-Newtonian behavior of the biopolymer.

Conclusions:

  • The study successfully identified an effective SCM treatment strategy for microbial dextran production.
  • Optimized fermentation conditions significantly enhanced dextran yield.
  • The produced dextran exhibits characteristics suitable for various industrial applications, with potential for further exploration.