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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

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Published on: March 16, 2011

Engineering microbial cell viability for enhancing chemical production by second codon engineering.

Liang Guo¹, Mengya Qi², Cong Gao¹

¹Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.

Metabolic Engineering

|August 20, 2022

Summary

This summary is machine-generated.

Engineering microbial cell factories to improve microbial cell viability (MCV) enhances industrial chemical production. By reducing reactive oxygen species (ROS), this strategy boosts yields for lysine and glucaric acid.

Keywords:

Engineering second codon Microbial cell factories Microbial cell viability ROS

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Published on: June 14, 2021

Area of Science:

Biotechnology
Synthetic Biology
Metabolic Engineering

Background:

Microbial cell factories are key for sustainable chemical production from biomass.
Poor microbial cell viability (MCV) limits their efficiency.
Reactive oxygen species (ROS) accumulation negatively impacts MCV.

Purpose of the Study:

To engineer MCV for enhanced industrial chemical production.
To reduce ROS accumulation by optimizing lifespan-specific gene regulation.
To demonstrate the efficacy of MCV engineering in different microbial hosts.

Main Methods:

Second codon engineering was employed to regulate key genes involved in ROS metabolism.
Hypoxia-inducible transcription factor (arcA) was targeted in Escherichia coli.
Ceramide synthase (lag1) was fine-tuned in Saccharomyces cerevisiae.

Main Results:

In E. coli, MCV improvement via arcA regulation led to lysine production of 213 g L^-1 at 5.90 g L^-1·h^-1.
In S. cerevisiae, MCV enhancement via lag1 tuning yielded glucaric acid production of 9.50 g L^-1 at 0.057 g L^-1·h^-1.
Reduced ROS accumulation correlated with improved MCV and chemical yields.

Conclusions:

Engineering microbial cell viability (MCV) is a viable strategy to enhance industrial chemical synthesis.
Optimizing gene regulation to minimize reactive oxygen species (ROS) is crucial for improving MCV.
This approach shows potential for boosting the performance of microbial cell factories in industrial applications.