Related Experiment Video
Updated: Aug 31, 2025
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
The clinical differences between cough variant asthma cells and humoral immunology indicators
Lei Jin1, Hua Gong1, Qinqin Zhang1
1Department of Clinical Laboratory, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi, 214000, China. yansong67103@163.com.
Insights
This study reveals elevated inflammatory markers and specific immune cell levels in children with cough variant asthma (CVA) during the attack stage. These immunological changes are crucial for diagnosing early CVA and preventing misdiagnosis.
Area of Science:
- Pediatric Immunology
- Respiratory Medicine
- Clinical Diagnostics
Background:
- Cough variant asthma (CVA) is a distinct phenotype of asthma characterized by chronic cough.
- Understanding the immunological underpinnings of CVA is crucial for accurate diagnosis and management.
- Previous research has not fully elucidated the specific cellular and humoral immune differences between CVA attack and remission stages.
Purpose of the Study:
- To compare clinical and immunological differences between children in the attack and remission stages of CVA and healthy controls.
- To analyze serum levels of key cytokines and humoral immunity indicators in different CVA stages.
- To assess the diagnostic significance of these immunological markers in early CVA detection.
Main Methods:
- A cohort of 73 children diagnosed with CVA was divided into attack (n=45) and remission (n=28) groups.
- A control group of 30 healthy children was included for comparison.
- Serum levels of tumor necrosis factor-alpha (TNF-α), high-sensitivity C-reactive protein (hs-CRP), interleukins (IL-4, IL-5, IL-6, IL-13), and T lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) were measured.
- Immunoglobulin (IgA, IgG, IgM, IgE) and IgG subclass levels (IgG1, IgG2, IgG3, IgG4) were also analyzed.
Main Results:
- Significantly elevated serum levels of TNF-α and hs-CRP were observed in the CVA attack group compared to remission and control groups (P<0.05).
- Interleukin levels (IL-4, IL-5, IL-6, IL-13) were significantly higher in the CVA attack stage (P<0.05).
- Elevated CD4+ T cells and CD4+/CD8+ ratios were noted in the attack stage (P<0.05), alongside significantly higher IgG4 subclass levels (P<0.05).
Conclusions:
- Cytokine, cellular, and humoral immune indicators in CVA patients deviate from normal ranges, indicating their involvement in CVA pathogenesis.
- Combined detection of these immunological markers holds significant clinical value for early CVA diagnosis.
- Identifying these markers can help avoid misdiagnosis and missed diagnoses of CVA in children.
Abstract:
The Purpose of this study was to study and analyze the clinical differences between cough variant asthma (CVA) cells and humoral immunology indicators. For this aim, 73 sick children with CVA were enrolled in this study and were admitted to the Pediatric Inpatient Department of Weifang Maternal and Child Health Hospital for treatment from April 2019 to May 2021. They were divided into the attack stage group (n=45) and the remission stage group (n=28). Meanwhile, 30 children with normal physical examination results were selected as normal controls. Differences in serum levels of TNF-, hs-CRP, IL-4, IL-5, IL-6 and IL-13 were compared among the three groups, as well as the differences in humoral immunology indicators such as T lymphocyte subsets CD3+, CD4+, CD8+, CD4+/ CD8+ and IgA, IgG, IgG, IgM, IgE, IgE and IgG subtypes. Results showed that serum levels of TNF-α and hs-CRP were significantly higher in the attack stage group than those in the remission stage group and normal control group, and the difference was statistically significant (P<0.05). The serum levels of TNF-α and hs-CRP were higher in the remission stage group than those in the normal control group, and the difference was not statistically significant (P>0.05). Serum levels of IL-4, IL-5, IL-6 and IL-13 were significantly higher in the attack stage group than those in the remission stage group and normal control group, and the difference was statistically significant (P<0.05). CD4+ and CD4+/CD8+ were significantly higher in the attack stage group than those in the remission stage group and normal control group, and the difference was statistically significant (P<0.05). The difference in serum levels of IgG1, IgG2 and IgG3 was not statistically significant (P>0.05) among the three groups. While the level of IgG4 subsets in the attack stage group was significantly higher than that in the remission stage group and normal control group, and the difference was statistically significant (P<0.05). Then the cytokines, cells and humoral immunology indicators of CVA patients are not in their normal range. They are involved in the pathogenesis of CVA. The combined detection is of great clinical significance in the diagnosis of early CVA to avoid misdiagnosis and missed diagnosis.
Related Concept Videos
01:26Asthma-II: Pathophysiology and Classification
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
01:20Asthma: Pathogenesis and Management
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
01:30Asthma-IV: Diagnostic and Management
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
01:29Asthma-I: Introduction
01:25Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
01:24Asthma-III: Symptoms and Complications
Classification of Asthma