Isotopic composition (δ¹³C and δ¹⁵N) in the soil-plant system of subtropical urban forests

This study brings information on the dynamics of C and N in urban forests in a subtropical region. We tested the hypothesis that C and N isotopic sign of leaves and soil and physiological traits of trees would vary from center to periphery in a megacity, considering land uses, intensity of automotive fleet and microclimatic conditions. 800 trees from four fragments were randomly chosen. Soil samples were collected at every 10 cm in trenches up to 1 m depth to analyze C and N contents. Both, plants and soil were assessed for δ¹³C, δ¹⁵N, %C and %N. Physiological traits [carbon assimilation (A)], CO₂ internal and external pressure ratio (Pi/Pa) and intrinsic water use efficiency iWUE were estimated from δ¹³C and Δ δ¹³C in leaves and soil ranged from -27.42 ‰ to -35.39 ‰ and from -21.22 ‰ to -28.18 ‰, respectively, and did not vary along the areas. Center-periphery gradient was not evidenced by C. Emissions derived from fossil fuel and distinct land uses interfered at different levels in δ¹³C signature. δ¹⁵N in the canopy and soil varied clearly among urban forests, following center-periphery gradient. Leaf δ¹⁵N decreased from the nearest forest to the city center to the farthest, ranging from <3 ‰ to <-3 ‰. δ¹⁵N was a good indicator of atmospheric contamination by NO_x emitted by vehicular fleet and a reliable predictor of land use change. %N followed the same trend of δ¹⁵N either for soils or leaves. Forest fragments located at the edges of the center-periphery gradient presented significantly lower A and Pi/Pa ratio and higher iWUE. These distinct physiological traits were attributed to successional stage and microclimatic conditions. Results suggest that ecosystem processes related to C and N and ecophysiological responses of urban forests vary according to land use and vehicular fleet.