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Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Related Experiment Video

Updated: Aug 31, 2025

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

Efficient bioparticle extraction using a miniaturized inertial microfluidic centrifuge.

Yaohui Fang¹, Shu Zhu¹, Weiqi Cheng¹

¹School of Mechanical Engineering, and Jiangsu Key Laboratory for Design, and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China. nan.xiang@seu.edu.cn.

|August 22, 2022

Summary

This summary is machine-generated.

This study introduces a miniaturized centrifuge using inertial microfluidics for efficient bioparticle extraction. The device offers a simple, portable alternative to traditional centrifuges, achieving high recovery rates for various cell types.

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Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Conventional bioparticle extraction methods are labor-intensive and require bulky, expensive centrifuges.
There is a need for simpler, more accessible methods for bioparticle isolation and concentration.

Purpose of the Study:

To develop and validate a miniaturized centrifuge for efficient bioparticle extraction from diverse biological samples.
To optimize the device's performance by analyzing channel dimensions and flow rates.
To demonstrate the device's versatility across multiple bioparticle isolation applications.

Main Methods:

Fabrication of a microfluidic device integrating four paralleled inertial spiral channels and a two-stage serpentine channel.
Experimental investigation of particle focusing dynamics and solution exchange efficiency.
Testing the device for cell culture medium exchange, cancer cell extraction, white blood cell purification, and magnetic microbead separation.

Main Results:

The miniaturized centrifuge achieved efficient washing and concentration of 10-20 μm particles with recovery efficiencies exceeding 93%.
Successful application in changing culture media for MCF-7 breast cancer cells.
Effective purification of A549 lung cancer cells, white blood cells (WBCs), and target cells from magnetic microbeads.

Conclusions:

The developed miniaturized centrifuge provides a simple, portable, and efficient alternative to conventional centrifugation.
The device avoids potential bioparticle damage associated with high-speed centrifugation.
It demonstrates broad applicability for bioparticle extraction from various biological fluids.