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Updated: Aug 31, 2025
Protocol to Create Chronic Wounds in Diabetic Mice
Published on: September 25, 2019
The Effect and Mechanism of External Use Ulcer Powder on Diabetic Mice
Bingyang Wang1, Wenlin Xie1, Xiaofang Lu1
1Department of Pathology, Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, CHN.
Abstract:
Objective Through the preparation of the diabetic mice skin ulcer model, we investigated the effect of Mongolian medicine external ulcer powder (WYK) on the treatment of diabetic skin ulcers and the expression of angiogenesis-related factors such as vascular endothelial growth factor (VEGF) and extracellular regulated protein kinases (ERK). Methods Thirty male clean Kunming mice were randomly divided into normal control group (group C), diabetic control group (group HC), and diabetic topical ulcer powder group (group HW). After successful modeling in the HC group and the HW group, the rats in the HW group were given external ulcer powder, which was applied to the back of the mice once a day. In addition, the rats in group C and group HC were treated with gentamicin injection external application once a day. The mice were sacrificed on the 3rd, the sixth, and the ninth day of dosing, and samples were taken. The adopted methods included protein immunoblotting (western blot) and reverse transcription-polymerase chain reaction (RT-PCR). The expression differences of angiogenesis-related factors such as VEGF and ERK in the repair process were detected. SPSS 13 software was used to analyze the results of angiogenesis-related factors VEGF and ERK. Results Comparison of VEGF and ERK Contents The serum VEGF content of mice in the HC group was significantly lower than that in the C group on days 3, 6, and 9 (p <0.05). The VEGF content in the HW group was significantly higher than that in the HC group (p <0.05). The content of ERK in serum was basically consistent with that of VEGF. The results of the western blot assay were consistent with those of the RT-PCR assay. Conclusion WYK can effectively promote the healing of skin ulcer wounds in diabetic mice, accelerate the proliferation of granulation tissue, enrich the contents of capillary blood tubes and collagen fibers, and increase the microvascular content. WYK can improve the expression level of VEGF and ERK in the serum of mice and advance the peak value of protein expression.
Insights
Mongolian medicine external ulcer powder (WYK) effectively treats diabetic skin ulcers in mice by promoting wound healing and increasing angiogenesis factors like vascular endothelial growth factor (VEGF) and extracellular regulated protein kinases (ERK).
Area of Science:
- Biomedical research
- Pharmacology
- Wound healing
Background:
- Diabetic skin ulcers present a significant clinical challenge due to impaired healing.
- Angiogenesis, crucial for wound repair, is often compromised in diabetic conditions.
- Traditional Mongolian medicine offers potential therapeutic avenues for complex wounds.
Purpose of the Study:
- To evaluate the efficacy of Mongolian medicine external ulcer powder (WYK) in treating diabetic skin ulcers in a mouse model.
- To investigate the impact of WYK on the expression of key angiogenesis factors, vascular endothelial growth factor (VEGF) and extracellular regulated protein kinases (ERK).
Main Methods:
- A diabetic mouse skin ulcer model was established.
- Mice were treated with WYK, gentamicin, or served as controls.
- Vascular endothelial growth factor (VEGF) and extracellular regulated protein kinases (ERK) expression were analyzed using western blot and RT-PCR.
- Statistical analysis was performed using SPSS software.
Main Results:
- Diabetic mice showed significantly lower serum VEGF and ERK levels compared to normal controls.
- WYK treatment significantly increased serum VEGF and ERK levels in diabetic mice compared to controls.
- WYK promoted granulation tissue proliferation, collagen fiber enrichment, and increased microvascular content.
Conclusions:
- Mongolian medicine external ulcer powder (WYK) effectively promotes the healing of diabetic skin ulcers.
- WYK enhances wound healing by increasing angiogenesis through elevated VEGF and ERK expression.
- WYK treatment positively influences tissue regeneration and vascularization in diabetic wounds.
