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Updated: Aug 31, 2025

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
Published on: August 20, 2015
Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
Gowher Ali1, Essam M Abdelalim2
1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), PO Box 34110, Doha, Qatar.
Abstract:
The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).
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