Related Experiment Video
Updated: Aug 31, 2025

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Functionalized monolithic columns: Recent advancements and their applications for high-efficiency separation and
Helong Si1, Quan Wang1,2,3, Yuanyuan Guo1,2,3
1College of Life Science, Hebei Agricultural University, Baoding, Hebei, China.
Abstract:
The chromatographic column is the core of a high-performance liquid chromatography (HPLC) system, and must have excellent separation efficiency and selectivity. Therefore, functional modification materials for monolithic columns have been rapidly developed. This study is a systematic review of the recently reported functionalized monolithic columns. In particular, the study reviews the types of functional monomers under different modification conditions, as well as the separation and detection techniques combined with chromatography, and their development prospects. In addition, the applications of functionalized monolithic columns in food analysis, biomedicine, and the analysis of active ingredient of Chinese herbal medicines in recent years are also discussed. Also reviewed are the functionalized monolithic columns for qualitative and quantitative analysis. It provided a reference for further development and application of organic polymer monolithic columns.
Related Concept Videos
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Principles Of Column Chromatography
Affinity Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...

