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Published on: April 18, 2025
The role of tetraspanins pan-cancer
Runzhi Huang1,2,3,4, Hanlin Sun1,5, Ruoyi Lin4
1Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou, 450052, China.
Abstract:
Tetraspanins (TSPANs) are a class of four-transmembrane segmented proteins. The precise functions of TSPANs and their roles in pan-cancer are unclear. In this work, we analyzed TSPAN1, TSPAN10, TSPAN11, TSPAN12, TSPAN13, TSPAN14, TSPAN15, TSPAN16, TSPAN17, TSPAN18, TSPAN18-AS1, TSPAN19, TSPAN2, TSPAN3, TSPAN31, TSPAN32, TSPAN33, TSPAN4, TSPAN5, TSPAN6, TSPAN7, TSPAN8, TSPAN9, TSPAN9-IT1 (24 TSPAN family genes) in relation to tumor characteristics from 11,057 TCGA samples across 33 cancer types. On 24 TSPAN family genes, multidimensional studies were conducted, including gene differential expression, immunological subtype analysis, clinical analysis, stemness indices analysis, drug sensitivity analysis, alteration analysis, and multi-omics validation (including ATAC-seq validation, single-cell sequencing validation, and other external validation). Genes were differentially expressed in 33 cancers, and several of them showed high consistency in terms of tumor characteristics. In particular, the potential roles of TSPAN15 and TSPAN1 in cancer deserve further attention.
Insights
Tetraspanins (TSPANs) are key proteins in cancer. This study analyzed 24 TSPAN genes across 33 cancers, revealing differential expression and potential roles for TSPAN15 and TSPAN1.
Area of Science:
- Molecular Biology
- Oncology
- Genomics
Background:
- Tetraspanins (TSPANs) are transmembrane proteins with poorly understood functions in cancer.
- Their specific roles across various cancer types remain largely unelucidated.
Purpose of the Study:
- To comprehensively analyze the role of 24 Tetraspanin family genes in pan-cancer.
- To investigate the relationship between TSPAN gene expression and tumor characteristics.
Main Methods:
- Utilized The Cancer Genome Atlas (TCGA) dataset comprising 11,057 samples across 33 cancer types.
- Conducted multidimensional analyses including differential gene expression, immune subtype, clinical, stemness, and drug sensitivity analyses.
- Performed multi-omics validation, including ATAC-seq and single-cell sequencing.
Main Results:
- Found differential expression of TSPAN genes across all 33 cancer types studied.
- Identified several TSPAN genes exhibiting consistent associations with tumor characteristics.
- Highlighted TSPAN15 and TSPAN1 as particularly noteworthy for their potential roles in cancer progression.
Conclusions:
- Tetraspanin gene family members display significant differential expression patterns in pan-cancer.
- TSPAN15 and TSPAN1 warrant further investigation for their potential as cancer biomarkers or therapeutic targets.
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