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Updated: Aug 31, 2025
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Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
Published on: February 11, 2021
7.2K
[Methods for the evaluation of intestinal mucosal permeability]
Jing-Ting Yan1,2, Qi Sun1, Xiao-Li Zhang1
1Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
Sheng Li Xue Bao : [Acta Physiologica Sinica]
|August 22, 2022
Summary
The intestinal mucosal barrier (IMB) protects the gut. This review summarizes IMB composition, function, and methods for evaluating intestinal mucosal permeability (IMP).
Area of Science:
- Gastroenterology
- Immunology
- Physiology
Background:
- The intestinal mucosal barrier (IMB) is crucial for maintaining gut integrity and preventing pathogen entry.
- The IMB comprises mechanical, chemical, biological, and immune components.
- Impaired IMB, indicated by increased intestinal mucosal permeability (IMP), is linked to various systemic diseases.
Purpose of the Study:
- To summarize the composition and function of the IMB.
- To review methods for evaluating IMP.
Main Methods:
- Electrophysiological recording of transmembrane resistance (TR) in ex vivo tissues or cell cultures.
- Biochemical quantification of transepithelial molecular probe or protein movement.
Main Results:
- The paper provides a comprehensive overview of IMB components and their roles.
- It details various techniques used to assess IMB function, focusing on IMP evaluation.
Conclusions:
- Understanding IMB composition and function is vital for diagnosing and managing related diseases.
- Accurate evaluation of IMP is essential for assessing IMB integrity.
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