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Updated: Jul 30, 2026

Influenza Virus Propagation in Embryonated Chicken Eggs
Published on: March 19, 2015
Apigenin inhibits infectious bronchitis virus replication in ovo
M J Saadh1, S A Jaber, M Alaraj
1Faculty of Pharmacy, Middle East University, Amman, Jordan. msaadeh@meu.edu.jo.
Objective:
Infectious bronchitis virus (IBV), for which no effective drugs are available, is among the most important causes of economic loss within the poultry industry. Apigenin is a flavonoid that can be isolated from plants. Apigenin has low toxicity with anti-viral activity. However, the effects of apigenin against IBV remain unclear.
Materials And Methods:
Thus, here we investigate the anti-viral effect of apigenin on IBV using 10 day-old embryonated eggs by determining the virus titer by embryo infective doses50 (EID50/mL) and determining IBV genomes copy number (per µL) of allantoic fluid.
Results:
We found that apigenin protected embryonated eggs from IBV. Additionally, apigenin reduced the log titer of the IBV with a significant correlation of up to 9.4 times at 2 µg/egg. Also, apigenin appears to significantly reduce IBV genomes copy number (per µL) in the allantoic fluid.
Conclusions:
Apigenin may be a promising approach for the treatment of IBV, since it protects embryonated eggs from IBV in ovo and suppresses viral replication.
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