nTiO₂ alleviates the toxic effects of TCPP on mussels by adjusting respiratory metabolism and gut microbiota

As a good carrier of contaminants, nanotitanium dioxide (nTiO₂) can absorb organic pollutants, producing toxicological effects on organisms. However, the complex effects of nTiO₂ with contaminants on marine mussels are still unclear. In this study, we exposed mussels to tris (2-chloropropyl) phosphate (TCPP) 100 μg/L (T1), 0.5 mg/L nTiO₂ + 100 μg/L TCPP (T2), 1.0 mg/L nTiO₂ + 100 μg/L TCPP (T3) and control (0 nTiO₂ + 0 μg/L TCPP) treatments, and assessed the combined effects of TCPP with nTiO₂ on the thick-shelled mussel Mytilus coruscus by detecting the activities of gill pyruvate kinase (PK), hexokinase (HK), lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH), also gill acetylcholine (Ach) and muscle lactic acid (LD) contents and gut microbiota after 14-d exposure. Compared with the control group, PK activity was increased significantly, but SDH, LDH activities and LD content were decreased significantly in T1, with the addition of nTiO₂, there were not significantly different in T3. However, Ach content in T3 was significantly higher than the control and T1. Moreover, KEGG of the gut microbiota via 16 s rRNA sequencing showed that most pathways returned to the control level in T3. The results showed that TCPP affected the respiratory metabolism of mussels, changed the community structure of intestinal microflora in mussels, and nTiO₂ alleviated the toxicity of TCPP. Our study provides new insights for ecological risk assessment of TCPP in bivalves in the complex aquatic environment and the novel role of nTiO₂ in regulating the toxicity of TCPP.