Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

784
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
784

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laryngoscope-Guided plasma radiofrequency ablation combined with incision/drainage for neonatal congenital pyriform Sinus Fistula with abscess: safety and minimally invasive approach.

Frontiers in pediatrics·2026
Same author

Correlation between the barrier function of the intestinal epithelium and susceptibility to PEDV infection in piglets at different ages.

Veterinary research·2026
Same author

Partial Discharge Gas Generation Characteristics and Molecular Degradation Mechanisms of Cellulose Polymers in Eco-Friendly Insulating Oils.

Polymers·2026
Same author

Immunogenicity and protective efficacy of an inactivated cluster 2.1 duck Tembusu virus vaccine in ducks: Evidence of cross-genotype immune responses.

Vaccine·2026
Same author

Unveiling HgS nanoparticle formation in Hg(II)-dissolved organic matter systems at low nanomolar to submicromolar levels: A comprehensive characterization via combined liquid chromatography-ICP-MS and single particle ICP-MS.

Journal of hazardous materials·2026
Same author

Mercury reduction in water by engineered silver nanoparticles (AgNPs): Effects of light irradiation and humic acid coating of AgNPs.

The Science of the total environment·2026

Related Experiment Video

Updated: Aug 31, 2025

Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut
09:13

Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut

Published on: January 31, 2025

561

Pigs' intestinal barrier function is more refined with aging.

Shanshan Yang1, Ning Yang2, Xin Huang3

  • 1State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China; Cell Biology and Immunology Group, Wageningen University and Research, Wageningen, the Netherlands.

Developmental and Comparative Immunology
|August 22, 2022
PubMed
Summary

Young piglets are highly susceptible to enteric virus infections due to their underdeveloped intestinal barriers and immature immune systems. This makes them vulnerable to viral invasion, leading to significant economic losses in swine farming.

Keywords:
AgesEnteric virusIntestinal environmentPigletsSusceptibility

More Related Videos

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
08:19

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Published on: February 10, 2023

2.6K
Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran
05:14

Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran

Published on: November 18, 2022

9.2K

Related Experiment Videos

Last Updated: Aug 31, 2025

Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut
09:13

Two-dimensional Porcine Intestinal Organoids Reflecting the Physiological Properties of Native Gut

Published on: January 31, 2025

561
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
08:19

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Published on: February 10, 2023

2.6K
Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran
05:14

Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran

Published on: November 18, 2022

9.2K

Area of Science:

  • Veterinary Virology
  • Animal Immunology
  • Gastroenterology

Background:

  • Enteric virus infections in piglets cause substantial economic losses.
  • Younger piglets exhibit higher susceptibility to these infections.
  • Understanding the reasons behind this susceptibility is crucial for disease control.

Purpose of the Study:

  • To investigate the causes of high susceptibility to enteric virus infections in young piglets.
  • To compare the intestinal barrier development in 1-week, 2-week, and 4-week-old piglets.
  • To identify specific factors contributing to increased viral vulnerability in neonatal swine.

Main Methods:

  • Histological staining to analyze intestinal villi morphology.
  • Real-time quantitative PCR (qPCR) to assess gene expression related to viral infection and immune cell differentiation.
  • Flow cytometry to measure T cell frequencies.

Main Results:

  • One-week-old piglets possess shallower intestinal villi crypts and less developed epithelial cells.
  • The immune system of 1-week-old piglets is significantly more immature compared to older piglets.
  • Early-weaned pigs show higher levels of viral invasion-assisting proteins and lower levels of resistant proteins.

Conclusions:

  • Immature intestinal barrier structure and function contribute to high enteric virus susceptibility in young piglets.
  • An underdeveloped immune system in neonatal swine exacerbates vulnerability to viral pathogens.
  • Targeting these factors could improve disease resistance and reduce economic impact in pig farming.