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MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and
S O Jaafar1, J O Jaffar2, S A Ibrahim2
1Department of Obstetrics and Gynecology, College of Medicine, Hawler Medical University, Erbil, Iraq.
Abstract:
Background: While different studies have investigated the association of SNPs with female reproductive disorders, a limited number of studies have investigated the effect of microRNAs variants in endometriosis. In this study, we evaluated the prevalence and the association of three different miRNAs variants including, miR-27a rs895819, miR-124-1 rs531564, and miR-423 rs6505162 with endometriosis to help further elucidate the importance of these variants in female reproductive disorders. Methods: A total number of 440 women (220 cases and 220 controls) were included. DNA was extracted and genotyping of the SNPs was carried out by PCR. Results: The results showed that rs895819 and rs6505162 had a significant association with endometriosis under the dominant, recessive, co-dominant, and allelic model, but rs531564 was not linked to endometriosis. Our results also imply a protective effect on endometriosis severity for AG genotype and G allele in rs895819 (p < 0.001), and also for AA and AC genotypes in rs6505162 with severity in endometriosis (p < 0.001). Moreover, Hardy-Weinberg equilibrium, haplotype frequency, and linkage disequilibrium between SNPs were performed. Conclusion: miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are linked to endometriosis.
Insights
MicroRNA variants miR-27a (rs895819) and miR-423 (rs6505162) are associated with endometriosis, potentially influencing disease severity. However, miR-124-1 (rs531564) showed no significant link to the condition.
Area of Science:
- Genetics
- Reproductive Biology
- Molecular Biology
Background:
- Endometriosis is a common gynecological disorder.
- Limited research exists on microRNA variants' role in endometriosis.
- Single nucleotide polymorphisms (SNPs) are increasingly studied in reproductive disorders.
Purpose of the Study:
- To investigate the association of three microRNA variants (miR-27a rs895819, miR-124-1 rs531564, miR-423 rs6505162) with endometriosis.
- To evaluate the prevalence and potential impact of these variants on disease severity.
- To elucidate the importance of microRNA variants in female reproductive disorders.
Main Methods:
- Genotyping of SNPs in 440 women (220 cases, 220 controls) using Polymerase Chain Reaction (PCR).
- DNA extraction and analysis of microRNA variants.
- Statistical analysis including Hardy-Weinberg equilibrium, haplotype frequency, and linkage disequilibrium.
Main Results:
- miR-27a (rs895819) and miR-423 (rs6505162) showed significant associations with endometriosis across various genetic models.
- miR-124-1 (rs531564) was not found to be linked to endometriosis.
- Specific genotypes and alleles of rs895819 and rs6505162 suggested a protective effect against endometriosis severity.
Conclusions:
- miR-27a rs895819 and miR-423 rs6505162 are significantly associated with endometriosis.
- miR-124-1 rs531564 is not linked to endometriosis.
- These findings highlight the potential role of specific microRNA variants in the pathogenesis and severity of endometriosis.
