Magnetic Relaxation Switch Sensor Based on Magnetophoresis and "T-Hg(II)-T" Signal Amplification

In this study, we designed a magnetic relaxation switch (MRS) sensor combined with magnetophoresis technology (MS-MRS), which helps solve the problems of traditional MRS sensors. The sensor is based on a new combined magnet and is composed of small magnetic blocks and iron sheets that can rapidly separate magnetic nanoparticles of different sizes within 5 min. The MS-MRS sensor consists of aptamer-functionalized magnetic nanoparticles (diameter: 200 nm) (MNP₂₀₀-Apt), complementary DNA-functionalized magnetic nanoparticles (diameter: 20 nm) (MNP₂₀-cDNA), and a combined magnet ("M2" magnet). The MNP₂₀₀-Apt probe could be separated by an "M2" magnet but the MNP₂₀-cDNA probe could not. To further improve the sensitivity of the sensor, we successfully constructed an MS-MRS-Hg sensor based on the "T-Hg(II)-T" specific recognition that aggregated MNP₂₀-cDNA probes to amplify the relaxation signal. The detection working range of the MS-MRS sensor is 0.5-100 ng/mL and that of the MS-MRS-Hg sensor is 0.05-100 ng/mL. Their limit of detection (LOD) values are 0.15 and 0.01 ng/mL, respectively. The relative recoveries of the MS-MRS and MS-MRS-Hg sensors are 95.2-119.5% and 93.1-113.1%, respectively. These results indicate that the proposed sensors have a high accuracy level.