Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Signaling in Plants01:25

Cell Signaling in Plants

5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
pH Regulation in Cells01:28

pH Regulation in Cells

6.5K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
6.5K
Stomach pH Regulation01:21

Stomach pH Regulation

6.2K
The human body carefully regulates the internal pH of different organs to maintain homeostasis. For example, while the blood plasma maintains a neutral pH of 7, the stomach lumen has an acidic pH of 1.5 - 3.5. The low pH of stomach lumen helps kill pathogens in the food and break down complex food molecules.
The acid-secreting gastric mucosal epithelial cells (parietal cells) lining the stomach lumen maintain the low pH in the lumen. Numerous ion transporters and channels on these parietal...
6.2K
Tonicity in Plants01:20

Tonicity in Plants

31.1K
Plant cells maintain appropriate osmotic balance in extreme conditions. For instance, plants in dry environments store water in vacuoles, limit the opening of their stoma, and have thick, waxy cuticles to prevent unnecessary water loss. Some species of plants that live in salty environments store salt in their roots. As a result, water osmosis occurs in the root from the surrounding soil.
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
31.1K
Responses to Salt Stress02:02

Responses to Salt Stress

13.3K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.3K
Photoreceptors and Plant Responses to Light02:00

Photoreceptors and Plant Responses to Light

23.0K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
23.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression and Characterization of a Recombinant Laccase with Alkalistable and Thermostable Properties from Streptomyces griseorubens JSD-1.

Applied biochemistry and biotechnology·2015
Same author

Herb-Partitioned Moxibustion and the miRNAs Related to Crohn's Disease: A Study Based on Rat Models.

Evidence-based complementary and alternative medicine : eCAM·2015
Same author

Bioactive carbazole alkaloids from the stems of Clausena lansium.

Fitoterapia·2015
Same author

Clauemarazoles A-G, seven carbazole alkaloids from the stems of Clausena emarginata.

Fitoterapia·2015
Same author

Scalable and DiI-compatible optical clearance of the mammalian brain.

Frontiers in neuroanatomy·2015
Same author

FSH regulates fat accumulation and redistribution in aging through the Gαi/Ca(2+)/CREB pathway.

Aging cell·2015

Related Experiment Video

Updated: Aug 31, 2025

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
09:46

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

Published on: April 28, 2014

15.2K

Extracellular pH sensing by plant cell-surface peptide-receptor complexes.

Li Liu1, Wen Song2, Shijia Huang3

  • 1Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology (SUSTech), Shenzhen, Guangdong 518055, China; Max-Planck Institute for Plant Breeding Research, Cologne 50829, Germany.

Cell
|August 23, 2022
PubMed
Summary

Plant peptide-receptor complexes sense extracellular pH, regulating root growth and immunity. Changes in pH alter peptide-receptor interactions, impacting plant development and defense responses.

Keywords:
Pep1RGF1alkalinizationextracellular pHpH-sensingpattern-triggered immunityplant peptidereceptor-like kinaseroot meristemsulfotyrosine

More Related Videos

Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator
11:54

Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

Published on: August 11, 2017

10.3K
In vitro Monitoring of Extracellular pH in Real-Time
10:11

In vitro Monitoring of Extracellular pH in Real-Time

Published on: June 3, 2021

1.9K

Related Experiment Videos

Last Updated: Aug 31, 2025

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
09:46

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

Published on: April 28, 2014

15.2K
Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator
11:54

Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

Published on: August 11, 2017

10.3K
In vitro Monitoring of Extracellular pH in Real-Time
10:11

In vitro Monitoring of Extracellular pH in Real-Time

Published on: June 3, 2021

1.9K

Area of Science:

  • Plant Biology
  • Molecular Plant Science
  • Plant Physiology

Background:

  • Extracellular pH is a critical regulator of plant biological processes.
  • The mechanisms by which plants perceive extracellular pH are not well understood.
  • Understanding pH sensing is crucial for deciphering plant growth and immunity regulation.

Purpose of the Study:

  • To investigate how plants perceive and respond to extracellular pH changes.
  • To elucidate the role of peptide-receptor complexes in pH sensing.
  • To understand the impact of pH sensing on root apical meristem (RAM) growth and immunity.

Main Methods:

  • Investigated the effect of pattern-triggered immunity (PTI) on extracellular pH in the RAM.
  • Analyzed the interaction between root meristem growth factor 1 (RGF1) and its receptors (RGFRs) under varying pH conditions.
  • Examined the binding of plant elicitor peptides (Peps) to their receptors (PEPRs) at different pH levels.
  • Performed domain swaps between RGFR and PEPR to assess functional pH dependency.

Main Results:

  • PTI induces extracellular alkalinization in the RAM, which is essential for RGF1-mediated growth.
  • Extracellular alkalinization inhibits the acidic-dependent RGF1-RGFR interaction via sulfotyrosine.
  • Extracellular alkalinization promotes the alkaline-dependent Pep-PEPR binding via Glu/Asp, enhancing immunity.
  • Domain swapping altered the pH sensitivity of RAM growth, confirming the role of receptor domains.

Conclusions:

  • Plant peptide-receptor complexes act as extracellular pH sensors.
  • Extracellular pH sensing by these complexes differentially regulates plant growth and immunity.
  • This mechanism provides insights into how plants integrate environmental pH cues for development and defense.