Determinants of carbon and nitrogen sequestration in multistrata agroforestry

Agroforestry has the potential to sequester carbon (C) and nitrogen (N), thereby counteracting climate change and soil degradation. However, the lack of empirical quantitative evidence on determinants of C and N stocks hampers the management of these stocks. The aim of this study was to identify the key determinants of the C and N stocks in multistrata agroforestry. We sampled 81 plots with broad variation in 12 hypothetical determinants of C and N stocks aboveground and in two soil layers, located in three Ethiopian regions with varied multistrata agroforestry traditions and characteristics. Above-ground stocks were assessed using an allometric equation, and soil stocks were assessed with the fixed-depth method. The hypothetical determinants, i.e., the duration of agroforestry practice, the tree density, the proportion of plants with a high basal diameter, legumes and native species, the species diversity, the main crops, soil texture and pH, and altitude, were tested using linear mixed models. The duration of agroforestry, tree density, and proportion of plants with a high basal diameter (>25 cm) explained half of the variation in the soil C stock, which represented nearly three quarters or more of the total C stock. Duration and tree density explained most of the soil N stock, although legumes also influenced soil N. A high proportion of large legume trees reduced C/N, whereas a high proportion of large non-legume trees increased C/N. The aboveground C stock was explained by species diversity or the proportion of stems with a basal diameter >10 cm, depending on the main crop. There is a high potential to manage C and N stocks and their persistence, as well as soil productivity, by managing the duration of agroforestry, the density of large trees, the proportion of legumes, and the main crops in multistrata agroforestry.