Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

4.9K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.9K
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

89
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
89
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.9K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LC3-associated phagocytosis in macrophage responses to Paracoccidioides spp.

Memorias do Instituto Oswaldo Cruz·2026
Same author

A novel viral RNA detection method based on the combined use of trans-acting ribozymes and HCR-FRET analyses.

PloS one·2024
Same author

Synergic Effect of the Antimicrobial Peptide ToAP2 and Fluconazole on <i>Candida albicans</i> Biofilms.

International journal of molecular sciences·2024
Same author

Editorial: Insights into fungal biology with emphasis on pathogenesis in humans.

Frontiers in fungal biology·2024
Same author

Applications of the Methylotrophic Yeast <i>Komagataella phaffii</i> in the Context of Modern Biotechnology.

Journal of fungi (Basel, Switzerland)·2024
Same author

Machine learning-based soil quality assessment for enhancing environmental monitoring in iron ore mining-impacted ecosystems.

Journal of environmental management·2024

Related Experiment Video

Updated: Aug 31, 2025

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
07:32

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Published on: March 19, 2015

10.1K

Cryptococcus neoformans Database in Synthetic Biology Open Language.

Sophia Garcia de Resende1, Ana Carolina Campos Batista1, Mayna da Silveira Gomide1,2

  • 1Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), DF, Brazil.

Microbiology Resource Announcements
|August 24, 2022
PubMed
Summary
This summary is machine-generated.

Synthetic biology tools can advance the study of Cryptococcus neoformans, a fungus causing lethal cryptococcosis. This research introduces a database of 23 essential genetic parts for engineering this important pathogen.

More Related Videos

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

64.5K
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

16.4K

Related Experiment Videos

Last Updated: Aug 31, 2025

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
07:32

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Published on: March 19, 2015

10.1K
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

64.5K
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

16.4K

Area of Science:

  • Mycology
  • Synthetic Biology
  • Genetics

Background:

  • Cryptococcus neoformans is a significant fungal pathogen responsible for cryptococcosis, a life-threatening global infection.
  • Understanding and manipulating C. neoformans is crucial for developing effective treatments and diagnostics.
  • Synthetic biology offers powerful approaches for dissecting microbial biology and function.

Purpose of the Study:

  • To address the challenge of limited genetic tools for Cryptococcus neoformans research.
  • To engineer genetic networks in C. neoformans for enhanced biological understanding.
  • To create a foundational resource of biological parts for synthetic biology applications in C. neoformans.

Main Methods:

  • Development and curation of a database of biological parts.
  • Characterization of 23 distinct genetic elements.
  • Standardization of parts using the Synthetic Biology Open Language (SBOL).

Main Results:

  • A novel database containing 23 standardized biological parts for C. neoformans was established.
  • These parts are compatible with synthetic biology workflows.
  • The database facilitates genetic engineering and network construction in C. neoformans.

Conclusions:

  • The developed database significantly expands the toolkit for synthetic biology in C. neoformans.
  • This resource will accelerate research into C. neoformans pathogenesis and enable the engineering of novel functions.
  • Accessible genetic parts are key to advancing the synthetic biology of medically important fungi.