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Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

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Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
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Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
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Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
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A Universal Method for Developing Autoinduction Expression Systems Using AHL-Mediated Quorum-Sensing Circuits.

Lai Li1,2, Aihua Deng1, Shuwen Liu1,3

  • 1CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

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|August 24, 2022
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Summary
This summary is machine-generated.

Researchers developed a universal method to screen quorum sensing (QS) systems for metabolic engineering. This approach enables dynamic regulation of gene expression, improving microbial production of desired products in bacteria.

Keywords:
autonomous inductionmetabolic engineeringprotein expressionquorum sensing

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Area of Science:

  • Microbial Engineering
  • Synthetic Biology
  • Metabolic Engineering

Background:

  • Maintaining a balance between cell growth and product synthesis is a key challenge in engineering microorganisms.
  • Quorum sensing (QS)-based dynamic regulations offer a pathway-independent method to control metabolic flux for biomass and product synthesis.
  • Current limitations include the lack of universal screening methods for QS elements and complex autoinduction circuit designs.

Purpose of the Study:

  • To develop a universal, simple, and rapid method for screening and evaluating QS systems in Gram-negative bacteria.
  • To construct and assess the largest library of QS combinations for metabolic engineering applications.
  • To establish a simple logical circuit for dynamic gene expression regulation to enhance protein and product synthesis.

Main Methods:

  • Developed a universal screening method for QS systems from Gram-negative bacteria.
  • Constructed and evaluated a library of 195 QS receiving device/signal molecule combinations in *Escherichia coli*.
  • Established a logical circuit with varying inducer synthesis rates for dynamic gene expression control.

Main Results:

  • Successfully screened and evaluated 195 QS combinations in *Escherichia coli*, creating the largest library to date.
  • Demonstrated efficient protein expression and product synthesis through dynamic regulation of gene expression.
  • Showcased the system's broad applicability by successfully applying it in *Pseudomonas putida*.

Conclusions:

  • The developed universal method simplifies and accelerates the screening of QS systems for metabolic engineering.
  • The established logical circuit enables dynamic control of gene expression, enhancing microbial biosynthesis.
  • This technology is adaptable to diverse Gram-negative strains, facilitating the production of desired compounds.