Related Experiment Video

Updated: Aug 31, 2025

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
10:07

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

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BCR-Associated Protein 31 Regulates Macrophages Polarization and Wound Healing Function via Early Growth Response

Qing Yuan1, Bo Zhao1, Yu-Hua Cao1

  • 1College of Life Science and Health, Northeastern University, Hunnan Xinqu, Shenyang, Liaoning, China.

Journal of Immunology (Baltimore, Md. : 1950)
|August 24, 2022
PubMed

Insights

BCR-associated protein 31 (BAP31) regulates macrophage function, suppressing M2 genes and affecting wound healing via IL-4Rα. This reveals a new mechanism for macrophage regulation and potential chronic wound treatments.

Area of Science:

  • Immunology
  • Cell Biology
  • Wound Healing Research

Background:

  • BCR-associated protein 31 (BAP31) is an endoplasmic reticulum transmembrane protein involved in immune cell regulation.
  • BAP31's role in macrophages remains largely unexplored, despite its known functions in microglia and T cells.

Purpose of the Study:

  • To investigate the function of BAP31 in macrophage polarization and activity.
  • To elucidate the molecular mechanisms by which BAP31 regulates macrophages.

Main Methods:

  • Generation of macrophage-specific BAP31 knockdown mice (Lyz2 Cre-BAP31flox/flox).
  • Analysis of macrophage polarization markers and gene expression.
  • Investigation of transcription factor regulation by BAP31, including C/EBPβ and early growth response 2.
  • Assessment of IL-4Rα ubiquitination and proteasome degradation.
  • Evaluation of macrophage functions (angiogenesis, skin fibrosis) in wound healing models, including IL-4Rα knockdown.

Main Results:

  • BAP31 knockdown suppressed M2 macrophage-associated genes.
  • BAP31 significantly decreased C/EBPβ expression, which is regulated by early growth response 2.
  • BAP31 modulates C/EBPβ by affecting IL-4Rα ubiquitination and proteasome degradation in IL-4-stimulated macrophages.
  • BAP31 influences macrophage functions in wound healing, including angiogenesis and skin fibrosis, mediated by IL-4Rα.

Conclusions:

  • BAP31 plays a novel role in regulating macrophage polarization and function.
  • BAP31 impacts macrophage-mediated wound healing processes through the IL-4Rα pathway.
  • These findings offer potential therapeutic targets for chronic wound treatment.

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