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Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for

John S H Danial^1,2, Jeff Y L Lam^3,4, Yunzhao Wu^3,4

¹Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK. js2494@cam.ac.uk.

Nature Protocols

|August 24, 2022

Summary

This summary is machine-generated.

Researchers developed NanoPro, an affordable and easy-to-build single-molecule localization microscopy (SMLM) system. This super-resolution imaging tool offers high performance, making advanced nanoscopy accessible to more scientists.

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Area of Science:

Biophysics
Optical Engineering
Medical Diagnostics

Background:

Single-molecule localization microscopy (SMLM) provides ultra-precise structural nanoscopy and sensitive medical diagnostics.
High cost and technical complexity limit SMLM accessibility for many life scientists.

Purpose of the Study:

To develop an economical, high-throughput, and easy-to-assemble SMLM instrument.
To democratize super-resolution imaging for biological and biomedical research.

Main Methods:

Construction of the NanoPro SMLM system using readily available components.
Development of a detailed, illustrated step-by-step protocol for assembly and validation.
Performance assessment using ground-truth samples down to 20 nm.

Main Results:

The NanoPro achieves performance comparable to high-end commercial SMLM systems.
It rivals existing open-source microscopes in quality but at lower cost and complexity.
Construction and validation are feasible for inexperienced researchers in under a week.

Conclusions:

The NanoPro significantly lowers the barrier to entry for super-resolution imaging.
It empowers broader adoption of SMLM in life sciences and medical diagnostics.
Facilitates faster progress in biological and biomedical discoveries.