Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Aug 31, 2025

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Published on: April 1, 2019

Novel composite trachea grafts using 3-dimensional printing.

Joanna F Weber^1,2, Sadiq S Rehmani³, Mirza Zain Baig^1,2

¹Rudy L. Ruggles Biomedical Institute, Nuvance Health, Danbury, Conn.

|August 25, 2022

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Can Artificial Intelligence Interpret Pulmonary Function Tests and Predict Prolonged Air Leaks After Lung Resection.

Cancers·2026

Same author

Lobar vs Sublobar Lung Resection for Early-Stage Epidermal Growth Factor Receptor-Mutant Non-Small Cell Lung Cancer.

The Annals of thoracic surgery·2026

Same author

Impact of Neoadjuvant Therapy, Tumor Size, and Surgical Approach on Conversion to Open Thoracotomy During Lobectomy.

The Annals of thoracic surgery·2026

Same author

Robotic Resection of an Ectopic Mediastinal Pancreatic Cyst.

Innovations (Philadelphia, Pa.)·2026

Same author

Evaluation of a powerless portable dialysate generator in a porcine model of continuous renal replacement therapy.

BMJ military health·2026

Same author

Thoracic Surgeons' Practice Patterns and Attitudes Toward Preoperative Testing for Blood Resource Use: An International Survey.

Annals of thoracic surgery short reports·2025

Small intestine submucosa (SIS) extracellular matrix (ECM) composite tracheal grafts showed good external integration but developed granulation tissue internally. Further research is needed to optimize SIS-ECM for airway graft applications.

Area of Science:

Biomaterials Science
Regenerative Medicine
Surgical Innovation

Background:

Porcine-derived small intestine submucosa (SIS) extracellular matrix (ECM) is proposed to have superior regenerative properties over dermal ECM.
Bioengineered composite grafts aim to improve tissue regeneration and integration in surgical applications.

Purpose of the Study:

To evaluate the incorporation of SIS-ECM in a bioengineered composite tracheal graft.
To compare the regenerative potential of SIS-ECM against dermal ECM in tracheal reconstruction.

Main Methods:

Two polycaprolactone (PCL) scaffold designs (rigid and flexible) were created for tracheal grafts.
SIS-ECM was applied to PCL scaffolds, forming composite grafts implanted in Yorkshire pigs.
Graft patency was assessed via CT scans, with gross and histological examination of explanted tissues.

Keywords:

3D, 3-dimensional 3D-printing CT, computed tomography ECM, extracellular matrix PCL, polycaprolactone SIS, small intestine submucosa extracellular matrix large animal model polycaprolactone small intestine submucosa tissue engineering trachea trachea graft trachea reconstruction

More Related Videos

Three-Dimensional Printing of a Complex Aortic Anomaly

Three-Dimensional Printing of a Complex Aortic Anomaly

Published on: November 1, 2018

Novel Process for 3D Printing Decellularized Matrices

Novel Process for 3D Printing Decellularized Matrices

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Aug 31, 2025

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Published on: April 1, 2019

Three-Dimensional Printing of a Complex Aortic Anomaly

Three-Dimensional Printing of a Complex Aortic Anomaly

Published on: November 1, 2018

Novel Process for 3D Printing Decellularized Matrices

Novel Process for 3D Printing Decellularized Matrices

Published on: January 7, 2019

Main Results:

All pigs survived the immediate postoperative period with generally good extraluminal graft incorporation.
Flexible grafts led to longer survival but severe intraluminal granulation tissue.
Rigid grafts showed less intraluminal granulation but experienced proximal anastomosis dehiscence.

Conclusions:

SIS-ECM demonstrates regenerative capabilities, evidenced by good extraluminal graft incorporation.
The current SIS-ECM substrate is not ideal for airway grafts due to intraluminal granulation tissue formation.
Modifications to the SIS-ECM substrate are necessary to enhance luminal integration and control granulation tissue development.