Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toxicokinetic Differences between Dermal and Oral Exposure to Ultraviolet Absorbers: Exploring Pathway-Specific Biomarkers.

Environmental science & technology·2026
Same author

Norepinephrine attenuates acute lung injury by protecting mitochondria via the β<sub>2</sub>-AR-AKAP1 axis and inhibiting alveolar epithelial pyroptosis.

Redox biology·2026
Same author

Regulating hydrogen-bond network via competitive coordination for cryogenic aqueous Zn-ion batteries.

Journal of colloid and interface science·2026
Same author

Impact of hepatitis B virus surface antigen mutations in the major hydrophilic region on antigen detectability.

Frontiers in cellular and infection microbiology·2026
Same author

Simultaneous determination of N-nitrosamines in ready-to-cook dishes: optimized extraction-purification protocol and comparative quantification by GC-MS/MS versus GC-Q/TOF MS.

Food research international (Ottawa, Ont.)·2026
Same author

Proteasome-haem-BACH2 axis fuels T cell exhaustion.

Trends in immunology·2026

Related Experiment Video

Updated: Aug 31, 2025

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

39.7K

Common methods in mitochondrial research (Review).

Yiyuan Yin1, Haitao Shen1

  • 1Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.

International Journal of Molecular Medicine
|August 25, 2022
PubMed
Summary

Mitochondrial abnormalities affect organ health through various mechanisms. This review details methods for detecting these changes, aiding disease diagnosis and treatment.

Keywords:
mitochondriamitochondrial DNAmitochondrial diseasesmitochondrial dysfunctionmitochondrial morphology

More Related Videos

Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
08:22

Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila

Published on: September 30, 2015

9.6K
Determination of Mitochondrial Morphology in Live Cells Using Confocal Microscopy
06:57

Determination of Mitochondrial Morphology in Live Cells Using Confocal Microscopy

Published on: July 3, 2025

285

Related Experiment Videos

Last Updated: Aug 31, 2025

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

39.7K
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
08:22

Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila

Published on: September 30, 2015

9.6K
Determination of Mitochondrial Morphology in Live Cells Using Confocal Microscopy
06:57

Determination of Mitochondrial Morphology in Live Cells Using Confocal Microscopy

Published on: July 3, 2025

285

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pathology

Background:

  • Mitochondrial abnormalities impact organ function (heart, brain, muscle) via oxidative stress, metabolic dysfunction, and genetic mutations.
  • Accurate detection of mitochondrial alterations is crucial for understanding and treating related diseases.

Purpose of the Study:

  • To review and highlight key methods for researching mitochondrial abnormalities.
  • To focus on mitochondrial extraction, purification, morphology, structure, and function analysis.

Main Methods:

  • Classical techniques: electron microscopy, mitochondrial membrane potential monitoring.
  • Modern approaches: ultrastructural determination, mtDNA mutation assays, metabolomics, regulatory mechanism analysis.

Main Results:

  • A comprehensive overview of established and emerging techniques for mitochondrial research.
  • Demonstration of how these methods facilitate accurate detection of mitochondrial dysfunction.

Conclusions:

  • Advanced methods improve the identification of mitochondrial abnormalities.
  • These detection techniques offer valuable guidance for diagnosing and treating diseases linked to mitochondrial dysfunction.