Related Experiment Video

Updated: Aug 31, 2025

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
08:18

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Published on: December 31, 2014

28.1K

Circ_0003489 facilitates multiple myeloma progression by targeting miR-433-3p/PBX3 axis

Tielun Yan1, Xiaoli Wang2, Dajin Zhou1

  • 1Department of Clinical Laboratory, Lishui Second People's Hospital, Lishui, People's Republic of China.

Hematology (Amsterdam, Netherlands)
|August 25, 2022
PubMed
Abstract

Insights

Circular RNA_0003489 (circ_0003489) promotes multiple myeloma (MM) progression by targeting the microRNA-433-3p (miR-433-3p)/Pre-B-cell leukemia homeobox 3 (PBX3) axis. This suggests circ_0003489 may be a potential therapeutic target for MM.

Area of Science:

  • Hematologic Oncology
  • Molecular Biology
  • Non-coding RNA Research

Background:

  • Multiple myeloma (MM) is a common hematologic malignancy.
  • Research into non-coding RNAs in MM is expanding.
  • The regulatory roles of circular RNAs in MM are increasingly investigated.

Purpose of the Study:

  • To elucidate the regulatory mechanism of the circ_0003489/miR-433-3p/PBX3 axis in multiple myeloma.
  • To investigate the functional role of circ_0003489 in MM progression.

Main Methods:

  • Quantification of circ_0003489, miR-433-3p, and PBX3 using RT-qPCR and Western blot.
  • Assessment of MM cell proliferation and apoptosis via CCK-8, flow cytometry, and EdU assays.
  • Confirmation of molecular interactions using dual-luciferase reporter and RNA pull-down assays.

Main Results:

  • Circ_0003489 and PBX3 were upregulated, while miR-433-3p was downregulated in MM tissues.
  • Circ_0003489 knockdown and miR-433-3p overexpression inhibited MM cell proliferation and induced apoptosis.
  • Circ_0003489 acts as a sponge for miR-433-3p, regulating PBX3 expression and MM progression.

Conclusions:

  • The circ_0003489/miR-433-3p/PBX3 axis drives multiple myeloma progression.
  • Circ_0003489 may serve as a potential therapeutic target for multiple myeloma treatment.
Keywords:
Circ_0003489PBX3miR-433-3pmultiple myeloma

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.0K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.4K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.3K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.5K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K