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Updated: Aug 31, 2025
07:31
A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
Published on: May 3, 2021
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SNHG9/miR-214-5p/SOX4 feedback loop regulates osteosarcoma progression.
Chu-Jie Bai1, Tian Gao2, Jia-Yong Liu2
1Department of Orthopedic Oncology, Peking University Cancer Hospital & Institute, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing, China.
Neoplasma
|August 25, 2022
Summary
Long non-coding RNA SNHG9 promotes osteosarcoma (OS) progression by regulating cell proliferation, migration, and invasion. Targeting the SNHG9/miR-214-5p/SOX4 feedback loop may offer a new therapeutic strategy for osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Osteosarcoma (OS) is an aggressive bone cancer.
- Long non-coding RNAs (lncRNAs) are critical regulators in biological processes, including cancer.
- The specific role of lncRNA SNHG9 in OS progression remains largely unknown.
Purpose of the Study:
- To investigate the expression and function of lncRNA SNHG9 in osteosarcoma.
- To elucidate the regulatory mechanism of SNHG9 in OS cell behaviors.
- To explore the potential of the SNHG9/miR-214-5p/SOX4 axis as a therapeutic target for OS.
Main Methods:
- Quantitative real-time PCR to assess SNHG9 expression in OS tissues and cells.
- Cell proliferation, migration, and invasion assays to evaluate the functional role of SNHG9.
- Western blot and luciferase reporter assays to confirm interactions between SNHG9, miR-214-5p, and SOX4.
- Analysis of the regulatory feedback loop involving SNHG9, miR-214-5p, and SOX4.
Main Results:
- SNHG9 was significantly overexpressed in OS tissues and cells.
- Downregulation of SNHG9 suppressed OS cell proliferation, migration, and invasion.
- SNHG9 acts as a molecular sponge for miR-214-5p, and SOX4 is a target of miR-214-5p.
- A feedback loop involving SNHG9, miR-214-5p, and SOX4 was identified, regulating OS progression.
Conclusions:
- SNHG9 overexpression is a key driver in osteosarcoma progression.
- The SNHG9/miR-214-5p/SOX4 feedback loop plays a crucial role in regulating OS cell proliferation, migration, and invasion.
- This regulatory axis represents a promising novel therapeutic target for osteosarcoma treatment.
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