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A Co-Printed Nanoslit Surface Plasmon Resonance Structure in Microfluidic Device for LMP-1 Detection
Shu-Cheng Lo1,2, Shao-Sian Li3, Wen-Fai Yang1
1Institute of Applied Mechanics, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10614, Taiwan.
Biosensors
|August 25, 2022
Summary
A new micro/nanostructure co-hot embossing technique enables seamless integration of localized surface plasmon resonance (SPR) sensors with microfluidic channels. This method simplifies sensor fabrication and demonstrates sensitive detection of refractive index changes and latent membrane protein (LMP-1).
Area of Science:
- Nanotechnology
- Microfluidics
- Biosensing
Background:
- Localized surface plasmon resonance (SPR) sensors offer high sensitivity for biomolecular detection.
- Integrating SPR sensors with microfluidic systems enhances analysis efficiency but often requires precise alignment.
- Existing fabrication methods can be complex and time-consuming.
Purpose of the Study:
- To develop a novel micro/nanostructure co-hot embossing technique for integrating SPR sensors into microfluidic channels.
- To demonstrate the alignment-free fabrication of SPR sensors within microfluidic devices.
- To validate the performance of the integrated system for refractive index sensing and protein quantification.
Main Methods:
- A micro/nanostructure co-hot embossing technique was employed to fabricate gold-capped nanostructure SPR sensors.
- The SPR sensors were integrated into microfluidic channels without the need for precise alignment during bonding.
- The sensitivity of the SPR sensor was characterized using varying concentrations of glycerol solutions.
- The SPR sensor was functionalized with anti-latent membrane protein 1 (anti-LMP-1) antibodies for specific protein detection.
Main Results:
- The co-hot embossing technique successfully integrated SPR sensors and microfluidic channels in an alignment-free manner.
- The SPR sensor demonstrated sensitivity to changes in refractive index, validated with glycerol solutions.
- The SPR sensor successfully quantified latent membrane protein 1 (LMP-1) across different sample concentrations.
- A calibration curve was established for LMP-1 quantification, demonstrating the system's potential for biomarker detection.
Conclusions:
- The novel micro/nanostructure co-hot embossing technique provides an efficient and simplified method for fabricating integrated SPR sensor and microfluidic systems.
- The developed system exhibits robust performance for both general refractive index sensing and specific biomarker quantification.
- This technology holds promise for developing advanced point-of-care diagnostic devices and sensitive bioanalytical tools.
