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Updated: Aug 31, 2025

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A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers.

Chih-Lang Lin1,2, Shyang-Guang Wang3, Meng-Tsung Tien4

  • 1Graduate Institute of Biotechnology and Biomedical Engineering, Central Taiwan University of Science and Technology, Taichung City 40601, Taiwan.

Biosensors
|August 25, 2022
PubMed
Summary

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This study introduces a new method using optical cell-tearing and dilution regulations to quantify cell surface antigen variations. This technique offers a novel approach for early disease diagnosis through antigen analysis.

Area of Science:

  • Biophysics
  • Biochemistry
  • Micro-/Nano-Engineering

Background:

  • Quantitative analysis of cell surface antigens is crucial for early disease diagnosis.
  • Antigenic variation recognition aids in understanding disease progression.

Purpose of the Study:

  • To present a novel methodology for detecting cell surface antigen variations.
  • To enable intuitive estimation of antigenic variation through relative-quantitative analysis.

Main Methods:

  • Utilizing optical "cell-tearing" with gradually reduced laser powers.
  • Employing "dilution regulations" with serial antibody dilutions to assay cell-binding firmness.
  • Experimenting with Groups B and B3 of red blood cells (RBCs).

Main Results:

Keywords:
agglutination testantigenic variationcell surface antigenscell-tearingoptical tweezerswhole-cell-based analysis

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  • A higher antibody dilution required lower laser power to detach cells.
  • Established a relative-quantitative analysis theory for estimating antigenic variation.
  • Demonstrated good consistency between estimated and literature findings.

Conclusions:

  • The proposed methodology offers a novel approach for examining cell surface antigen variations.
  • The technique is expected to have broad applications in sensor development.
  • Potential applications span biochemistry, biophysics, and micro-/nano-engineering fields.