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Updated: Aug 31, 2025
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Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
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Dynamin-Related Proteins Enhance Tomato Immunity by Mediating Pattern Recognition Receptor Trafficking
Meirav Leibman-Markus1,2, Silvia Schuster1, Beatriz Vasquez-Soto3
1School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6997801, Israel.
Membranes
|August 25, 2022
Summary
Dynamin-related protein SlDRP1B regulates pattern recognition receptor trafficking, enhancing plant immunity. This protein modifies LeEIX2 endocytosis and boosts defense responses, similar to SlDRP2A.
Area of Science:
- Plant molecular biology
- Plant immunity
- Cellular trafficking
Background:
- Pattern recognition receptor (PRR) trafficking and endocytosis are vital for plant immunity.
- Dynamin-related proteins (DRPs) are involved in endocytosis and recycling.
- SlDRP2A in tomato regulates PRR LeEIX2 endocytosis and immunity.
Purpose of the Study:
- To investigate the function of SlDRP1B, a tomato DRP2 orthologue, in PRR trafficking and plant immunity.
- To determine if SlDRP1B participates in processes similar to SlDRP2A.
Main Methods:
- Bioinformatic selection of SlDRP1B among tomato DRP2 orthologues.
- Transient expression of SlDRP1B and its dominant-negative mutant in *Nicotiana* species.
- Analysis of SlDRP1B association with LeEIX2 and its effect on LeEIX2 trafficking.
Main Results:
- SlDRP1B physically associates with the PRR LeEIX2, increasing its presence in endosomes.
- SlDRP1B enhances EIX-elicited defense responses.
- Overexpression of SlDRP1B also enhances flg22-elicited defense responses.
Conclusions:
- SlDRP1B plays a role in regulating PRR trafficking.
- SlDRP1B contributes to plant immunity, similar to SlDRP2A.
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